ArticleNucleic acids research2017
Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Companion cells with high florigen production express other small proteins and reveal a nitrogen-sensitiveeLife · 2025Article
- Companion cells with high florigen production express other small proteins and reveal a nitrogen-sensitivebioRxiv : the preprint server for biology · 2025Article
- Unveiling the Genomic Symphony: Identification Cultivar-Specific Genes and Enhanced Insights on Sweet Sorghum Genomes Through Comprehensive superTranscriptomic Analysis.Journal of molecular evolution · 2024Article
- Enhancer activation via TCP and HD-ZIP and repression by Dof transcription factors mediate giant cell-specific expression.The Plant cell · 2023Article
- Evaluating EcxR for Its Possible Role inInternational journal of molecular sciences · 2022Article
- A genome-scale TF-DNA interaction network of transcriptional regulation of Arabidopsis primary and specialized metabolism.Molecular systems biology · 2021Article
- Identification of transcription factors that regulateAutophagy · 2020Article
- Article
- The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat.BMC plant biology · 2019Article
- High-Throughput Yeast One-Hybrid Screens Using a Cell Surface gLUC Reporter.Current protocols in plant biology · 2019Article
- Functional dissection of thePlant direct · 2019Article
- ThePlant physiology · 2018Article
- Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.Zebrafish · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.
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Registered trials
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