ArticleJBRA assisted reproduction2017
Influence of the abstinence period on human sperm quality: analysis of 2,458 semen samples.
Article in JBRA assisted reproduction, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 3 syntheses or guidelines pooled it, 65 citations in OpenAlex.
- Impact of short abstinence versus testicular sperm on sperm DNA fragmentation: a systematic review and meta-analysis.Archives of gynecology and obstetrics · 2024Pooled it
- Effects of long and short ejaculatory abstinence on sperm parameters: a meta-analysis of randomized-controlled trials.Frontiers in endocrinology · 2024Pooled it
- The Effect of Male Sexual Abstinence Periods on the Clinical Outcomes of Fresh Embryo Transfer Cycles Following Assisted Reproductive Technology: A Meta-Analysis.American journal of men's healthPooled it
- Physiological basis of contraceptive function of a GSK3α-selective inhibitor, manifested during epididymal sperm maturation.Molecular human reproduction · 2026Article
- Influence of Very Short-Term Sexual Abstinence on Basic Semen Parameters and Sperm Chromatin Status: A Preliminary Study.International journal of molecular sciences · 2026Article
- Article
- Case Report: Reappearance of spermatozoa following multicomponent lifestyle modification in men with previously diagnosed azoospermia: a case series.Frontiers in urology · 2026Article
- Association of sperm DNA fragmentation with abstinence duration, seminal oxidative stress and age: a call to consider abstinence in andrological diagnostics.Frontiers in urology · 2026Article
- Seminal dysbiosis in idiopathic male infertility: toward a seminal dysbiosis-inflammatory/oxidative endophenotype-SDF axis.Frontiers in endocrinology · 2026Review
- The role of sperm DNA fragmentation in reproduction: a SWOT analysis.Journal of assisted reproduction and genetics · 2025Review
- Impact of Shorter Abstinence Periods on Semen Parameters: A Systematic Review and Meta-Analysis.The world journal of men's health · 2025Article
- Ejaculatory abstinence and its impacts on within- and between-individual variations in semen parameters of 9,595 Vietnamese men.Translational andrology and urology · 2025Article
- Association of abstinence time with semen quality in men who undergoing fertility evaluation: a cross-sectional study from 3052 participants.Frontiers in endocrinology · 2025Article
- Sperm DNA Fragmentation: causes, evaluation and management in male infertility.JBRA assisted reproduction · 2024Review
- Article
- Association between penultimate ejaculatory abstinence and sperm quality: a cross-sectional study.Frontiers in endocrinology · 2024Article
- Effects of a short abstinence period on sperm quality in oligozoospermic men.Clinical and experimental reproductive medicine · 2023Article
- Can a sperm selection technique improve embryo ploidy?Andrology · 2023Article
- The Influence of Male Ejaculatory Abstinence Time on Pregnancy Rate, Live Birth Rate and DNA Fragmentation: A Systematic Review.Journal of clinical medicine · 2023Review
- Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men-How Does the Seminal Bacteriome Respond?International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation.
methodsWe recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded. Associations between the sexual abstinence period and sperm parameters were assessed using Spearman correlation. For group comparisons, the subjects were categorized according to the sexual abstinence period (SAP) into three groups: SAP <2 days, SAP 2-5 days, and SAP >5 days.
resultsThe duration of abstinence had a statistically significant positive influence on sperm concentration and volume, the number of leukocytes and a statistically significant negative influence on sperm motility and vitality. The percentages of DNA fragmentation and MMP (mitochondrial damage) worsened with the increased duration of abstinence. The percentage of sperm protamination was statistically significantly increased with abstinence.
conclusionIncrease in the sexual abstinence period influences sperm quality. This study reinforces the importance of the duration of ejaculatory abstinence on semen parameter variation. It highlights the deleterious effect of increased abstinence on DNA damage, which is most likely associated with ROS (mitochondrial damage/number of leukocytes). The increase in chromatin packaging can represent a protective feature for DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.