ArticleObesity (Silver Spring, Md.)2017
Comparison of Methods for Analyzing Human Adipose Tissue Macrophage Content.
Article in Obesity (Silver Spring, Md.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- The age of obesity onset affects changes in subcutaneous adipose tissue macrophages and T cells after weight loss.Frontiers in immunology · 2025Article
- Adipose Tissue Analysis Toolkit (ATAT) for automated analysis of adipocyte size and extracellular matrix in white adipose tissue.Obesity (Silver Spring, Md.) · 2024Article
- Macrophage Phenotypes and Gene Expression Patterns Are Unique in Naturally Occurring Metabolically Healthy Obesity.International journal of molecular sciences · 2022Article
- Article
- Chronic ATAmerican journal of physiology. Endocrinology and metabolism · 2021Article
- Crown-like structures in breast adipose tissue of breast cancer patients: associations with CD68 expression, obesity, metabolic factors and prognosis.NPJ breast cancer · 2021Article
- Adipose tissue macrophage populations and inflammation are associated with systemic inflammation and insulin resistance in obesity.American journal of physiology. Endocrinology and metabolism · 2021Article
- Adipose tissue macrophage burden, systemic inflammation, and insulin resistance.American journal of physiology. Endocrinology and metabolism · 2020Article
- Senolytics decrease senescent cells in humans: Preliminary report from a clinical trial of Dasatinib plus Quercetin in individuals with diabetic kidney disease.EBioMedicine · 2019 · on this mapArticle
- Preliminary evidence for reduced adipose tissue inflammation in vegetarians compared with omnivores.Nutrition journal · 2019Article
- Immune Cells Gate White Adipose Tissue Expansion.Endocrinology · 2019Review
- Automated macrophage counting in DLBCL tissue samples: a ROF filter based approach.Biological procedures online · 2019Article
- Emerging Roles for Adipose Tissue in Cardiovascular Disease.Arteriosclerosis, thrombosis, and vascular biology · 2018Review
- Parallels in Immunometabolic Adipose Tissue Dysfunction with Ageing and Obesity.Frontiers in immunology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveThe relationship between inflammation, obesity, and adverse metabolic conditions is associated with adipose tissue macrophages (ATM). This study compared the measurements of human ATM using flow cytometry, immunohistochemistry (IHC), and real-time polymerase chain reaction (RT-PCR) of ATM markers.
methodsA new software program (AMCounter) was evaluated to help measure ATM using IHC, and this was compared to flow cytometry and RT-PCR.
resultsIHC had good intraindividual reproducibility for total (CD68), proinflammatory (CD14), and anti-inflammatory (CD206) ATM. The AMCounter improved interreader agreement and was more time efficient. Flow cytometry had acceptable intraindividual reproducibility for the percentage of CD68
conclusionsThe AMCounter software helps provide reproducible and efficient measures of IHC ATMs. Flow cytometry, IHC, and RT-PCR measures of adipose inflammation provide somewhat different information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.