ArticleJournal of cachexia, sarcopenia and muscle2018
miR-422a suppresses SMAD4 protein expression and promotes resistance to muscle loss.
Article in Journal of cachexia, sarcopenia and muscle, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Preoperative visceral fat and muscle loss after cardiovascular surgery: A retrospective cohort study.JPEN. Journal of parenteral and enteral nutrition · 2026Article
- IL-18 Binding Protein, a biomarker of strength maintenance after surgery but reduced physical performance in age-related sarcopenia.PloS one · 2026Article
- Mitochondrial Dysfunction Contributes to Sustained Muscle Loss After Cardiac Surgery: A Prospective Observational Study.Journal of cachexia, sarcopenia and muscle · 2025Observational
- The Role of miRNAs and Extracellular Vesicles in Adaptation After Resistance Exercise: A Review.Current issues in molecular biology · 2025Review
- Acute Sarcopenia: Mechanisms and Management.Nutrients · 2024Review
- Review
- Acute Sarcopenia after Elective and Emergency Surgery.Aging and disease · 2022Review
- Review
- Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study.Pathophysiology : the official journal of the International Society for Pathophysiology · 2022Article
- Biomarkers for cancer cachexia: where do we stand?Journal of cachexia, sarcopenia and muscle · 2020Article
- Metabolic profiling shows pre-existing mitochondrial dysfunction contributes to muscle loss in a model of ICU-acquired weakness.Journal of cachexia, sarcopenia and muscle · 2020Article
- Review
- Identification of microRNAs in skeletal muscle associated with lung cancer cachexia.Journal of cachexia, sarcopenia and muscle · 2020Article
- Regulation of Skeletal Muscle Atrophy in Cachexia by MicroRNAs and Long Non-coding RNAs.Frontiers in cell and developmental biology · 2020Review
- miR-1-5p targets TGF-βR1 and is suppressed in the hypertrophying hearts of rats with pulmonary arterial hypertension.PloS one · 2020Article
- The Journal of Cachexia, Sarcopenia and Muscle stays the front-runner in geriatrics and gerontology.Journal of cachexia, sarcopenia and muscle · 2019Article
- Skeletal muscle miR-34a/SIRT1:AMPK axis is activated in experimental and human non-alcoholic steatohepatitis.Journal of molecular medicine (Berlin, Germany) · 2019Article
- Quadriceps miR-542-3p and -5p are elevated in COPD and reduce function by inhibiting ribosomal and protein synthesis.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- MicroRNAs in muscle wasting.Journal of cachexia, sarcopenia and muscle · 2018Article
- Nucleic Acid-Based Therapeutics for Pulmonary Diseases.AAPS PharmSciTech · 2018Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundLoss of muscle mass and strength are important sequelae of chronic disease, but the response of individuals is remarkably variable, suggesting important genetic and epigenetic modulators of muscle homeostasis. Such factors are likely to modify the activity of pathways that regulate wasting, but to date, few such factors have been identified.
methodsThe effect of miR-422a on SMAD4 expression and transforming growth factor (TGF)-β signalling were determined by western blotting and luciferase assay. miRNA expression was determined by qPCR in plasma and muscle biopsy samples from a cross-sectional study of patients with chronic obstructive pulmonary disease (COPD) and a longitudinal study of patients undergoing aortic surgery, who were subsequently admitted to the intensive care unit (ICU).
resultsmiR-422a was identified, by a screen, as a microRNA that was present in the plasma of patients with COPD and negatively associated with muscle strength as well as being readily detectable in the muscle of patients. In vitro, miR-422a suppressed SMAD4 expression and inhibited TGF-beta and bone morphogenetic protein-dependent luciferase activity in muscle cells. In male patients with COPD and those undergoing aortic surgery and on the ICU, a model of ICU-associated muscle weakness, quadriceps expression of miR-422a was positively associated with muscle strength (maximal voluntary contraction r = 0.59, P < 0.001 and r = 0.51, P = 0.004, for COPD and aortic surgery, respectively). Furthermore, pre-surgery levels of miR-422a were inversely associated with the amount of muscle that would be lost in the first post-operative week (r = -0.57, P < 0.001).
conclusionsThese data suggest that differences in miR-422a expression contribute to the susceptibility to muscle wasting associated with chronic and acute disease and that at least part of this activity may be mediated by reduced TGF-beta signalling in skeletal muscle.
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