ArticleInfection2017
Performance of automated multiplex PCR using sonication fluid for diagnosis of periprosthetic joint infection: a prospective cohort.
Article in Infection, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Diagnostic accuracy of sonication fluid cultures from prosthetic components in periprosthetic joint infection: an updated diagnostic meta-analysis.Journal of orthopaedic surgery and research · 2023Pooled it
- Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections.BMC microbiology · 2020Trial
- Mid- to Long-Term Outcomes of Two-Stage Revision Arthroplasty for Periprosthetic Joint Infection of the Shoulder.Journal of clinical medicine · 2025Article
- Synovial fluid leukocytes as diagnostic marker in periprosthetic shoulder infection.JSES international · 2025Article
- The identification of low-pathogenic bacteria on removed spinal implants and implications for antimicrobial prophylaxis.Brain & spine · 2025Article
- Improving the microbiological diagnosis of fracture-related infection and prosthetic joint infection through culturing sonication fluid in Bactec blood culture bottles.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Practical Guidance for Clinical Microbiology Laboratories: Microbiologic diagnosis of implant-associated infections.Clinical microbiology reviews · 2024Review
- Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology.The Journal of molecular diagnostics : JMD · 2023Review
- The role of serum D-Dimer for the diagnosis of periprosthetic shoulder infection.Archives of orthopaedic and trauma surgery · 2023Article
- Comparison of the BioFire Joint Infection Panel to 16S Ribosomal RNA Gene-Based Targeted Metagenomic Sequencing for Testing Synovial Fluid from Patients with Knee Arthroplasty Failure.Journal of clinical microbiology · 2022Article
- The role of serum C-reactive protein in the diagnosis of periprosthetic shoulder infection.Archives of orthopaedic and trauma surgery · 2022Article
- The DendrisCHIPDiagnostics (Basel, Switzerland) · 2022Article
- Sonication of explants enhances the diagnostic accuracy of synovial fluid and tissue cultures and can help determine the appropriate antibiotic therapy for prosthetic joint infections.International orthopaedics · 2022Article
- Diagnostic accuracy of multiplex polymerase chain reaction on tissue biopsies in periprosthetic joint infections.Scientific reports · 2021Article
- Direct detection of microorganisms in sonicated orthopedic devices after in vitro biofilm production and different processing conditions.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2021Article
- Preoperative synovial fluid culture poorly predicts the pathogen causing periprosthetic joint infection.Infection · 2021Article
- Ruling out underlying infection in 200 presumed aseptic knee and hip revision arthroplasties using a multiplex PCR system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- An update about molecular biology techniques to detect orthopaedic implant-related infections.EFORT open reviews · 2021Review
- Diagnosis of orthopaedic-implant-associated infections caused by slow-growing Gram-positive anaerobic bacteria - a clinical perspective.Journal of bone and joint infection · 2021Review
- Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSonication of explanted prostheses improved the microbiological diagnosis of periprosthetic joint infections (PJI). We evaluated the performance of automated multiplex polymerase chain reaction (PCR) using sonication fluid for the microbiological diagnosis of PJI.
methodsIn a prospective cohort using uniform definition criteria for PJI, explanted joint prostheses were investigated by sonication and the resulting sonication fluid was analyzed by culture and multiplex PCR. McNemar's Chi-squared test was used to compare the performance of diagnostic tests.
resultsAmong 111 patients, PJI was diagnosed in 78 (70%) and aseptic failure in 33 (30%). For the diagnosis of PJI, the sensitivity and specificity of periprosthetic tissue culture was 51 and 100%, of sonication fluid culture 58 and 100%, and of sonication fluid PCR 51 and 94%, respectively. Among 70 microorganisms, periprosthetic tissue culture grew 52 (74%), sonication fluid culture grew 50 (71%) and sonication fluid PCR detected 37 pathogens (53%). If only organisms are considered, for which primers are included in the test panel, PCR detected 37 of 58 pathogens (64%). The sonication fluid PCR missed 19 pathogens (predominantly oral streptococci and anaerobes), whereas 7 additional microorganisms were detected only by PCR (including Cutibacterium spp. and coagulase-negative staphylococci).
conclusionsThe performance of multiplex PCR using sonication fluid is comparable to culture of periprosthetic tissue or sonication fluid. The advantages of PCR are short processing time (< 5 h) and fully automated procedure. However, culture technique is still needed due to the low sensitivity and the need of comprehensive susceptibility testing. Modification of primers or inclusion of additional ones may improve the performance of PCR, especially of low-virulent organisms.
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