ArticleOptica2016
Selective two-photon collagen crosslinking
Article in Optica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Nanomaterial-Enhanced Corneal Cross-Linking: Engineering Strategies for Transforming Keratoconus Management.Pharmaceutics · 2026Review
- Assessing UVA and Laser-Induced Crosslinking via Brillouin Microscopy.Journal of biophotonics · 2025Article
- Optomechanical assessment of photorefractive corneal cross-linking via optical coherence elastography.Frontiers in bioengineering and biotechnology · 2023Article
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Ocular Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Nonlinear optical crosslinking (NLO CXL) for correcting refractive errors.Experimental eye research · 2020Review
- Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging.Scientific reports · 2019Article
- Coupling Microfluidic Platforms, Microfabrication, and Tissue Engineered Scaffolds to Investigate Tumor Cells Mechanobiology.Micromachines · 2019Review
- Spatially-resolved Brillouin spectroscopy reveals biomechanical abnormalities in mild to advanced keratoconus in vivo.Scientific reports · 2019Article
- Morpho-mechanics of human collagen superstructures revealed by all-optical correlative micro-spectroscopies.Communications biology · 2019Article
- Brillouin microscopy: assessing ocular tissue biomechanics.Current opinion in ophthalmology · 2018Review
- Effects of Corneal Hydration on Brillouin Microscopy In Vivo.Investigative ophthalmology & visual science · 2018Article
- Flexible Optical Waveguides for Uniform Periscleral Cross-Linking.Investigative ophthalmology & visual science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Two-photon polymerization has enabled precise microfabrication of three-dimensional structures with applications spanning from photonic microdevices, drug delivery systems, and cellular scaffolds. We present two-photon collagen crosslinking (2P-CXL) of intact corneal tissue using riboflavin and femtosecond laser irradiation. Collagen fiber orientations and photobleaching were characterized by second harmonic generation and two-photon fluorescence imaging, respectively. Measurement of local changes in longitudinal mechanical moduli with confocal Brillouin microscopy enabled the visualization of the cross-linked pattern without perturbation of the surrounding non-irradiated regions. 2P-CXL induced stiffening was comparable to that achieved with conventional one-photon CXL. Our results demonstrate the ability to selectively stiffen biological tissue
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.