ArticleFrontiers in microbiology2017
A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host Cells
Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Beyond nocardioform: Transcriptionally active microbes and host responses in equine mucoid placentitis.Equine veterinary journal · 2026Article
- CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.Nature communications · 2026Article
- Optimal Dual RNA-Seq Mapping for Accurate Pathogen Detection in Complex Eukaryotic Hosts.Bio-protocol · 2025Article
- Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases.Veterinary sciences · 2024Review
- Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection.Applied microbiology and biotechnology · 2023Article
- dRNASb: a systems biology approach to decipher dynamics of host-pathogen interactions using temporal dual RNA-seq data.Microbial genomics · 2022Article
- Reducing costs for DNA and RNA sequencing by sample pooling using a metagenomic approach.BMC genomics · 2022Article
- Steroid-Refractory Gut Graft-Versus-Host Disease: What We Have Learned From Basic Immunology and Experimental Mouse Model.Frontiers in immunology · 2022Review
- Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus.Scientific reports · 2021Article
- Omics in gut microbiome analysis.Journal of microbiology (Seoul, Korea) · 2021Review
- Dual RNASeq Reveals NTHi-Macrophage Transcriptomic Changes During Intracellular Persistence.Frontiers in cellular and infection microbiology · 2021Article
- Article
- Spatially distinct physiology of Bacteroides fragilis within the proximal colon of gnotobiotic mice.Nature microbiology · 2020Article
- Article
- A survey ofMicrobial genomics · 2020Article
- Transcriptional Profiling of Host Cell Responses to VirulentInternational journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the same biological sample, dual RNA-Seq can provide unique insight into bacterial infection processes and reciprocal host responses at once. However, the difficulties involved in handling both prokaryotic and eukaryotic material require distinct, optimized procedures. We previously developed and applied dual RNA-Seq to measure prokaryotic and eukaryotic expression profiles of human cells infected with bacteria, using
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.