ArticleNew journal of chemistry = Nouveau journal de chimie2017
Solution-phase and solid-phase sequential, selective modification of side chains in KDYWEC and KDYWE as models for usage in single-molecule protein sequencing.
Article in New journal of chemistry = Nouveau journal de chimie, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A generalized protein identification method for novel and diverse sequencing technologies.NAR genomics and bioinformatics · 2024Article
- Amplifiable protein identification via residue-resolved barcoding and composition code counting.National science review · 2024Article
- Review
- Single-molecule fluorescence methods for protein biomarker analysis.Analytical and bioanalytical chemistry · 2023Review
- Evaluating the Effect of Dye-Dye Interactions of Xanthene-Based Fluorophores in the Fluorosequencing of Peptides.Bioconjugate chemistry · 2022Article
- Protein Sequencing, One Molecule at a Time.Annual review of biophysics · 2022Review
- Cataloguing the proteome: Current developments in single-molecule protein sequencing.Biophysics reviews · 2022Review
- Studies of Surface Preparation for the Fluorosequencing of Peptides.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Photoredox-Catalyzed DecarboxylativeACS chemical biology · 2021Article
- The emerging landscape of single-molecule protein sequencing technologies.Nature methods · 2021Review
- Strategies for Development of a Next-Generation Protein Sequencing Platform.Trends in biochemical sciences · 2020Review
- Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore.ACS nano · 2019Article
- FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution.Nature communications · 2019Article
- Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures.Nature biotechnology · 2018Article
- Paving the way to single-molecule protein sequencing.Nature nanotechnology · 2018Review
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Authors and funding
4 authors.
Funding
Abstract
Single-molecule protein sequencing is regarded as a promising new method in the field of proteomics. It potentially offers orders of magnitude improvements in sensitivitiy and throughput for protein detection when compared to mass spectrometry. However, the development of such a technology faces significant barriers, especially in the need to chemically derivatize specific amino-acid types with unique labels. For example, fluorescent dyes would be suitable for single-molecule microscopy or nanopore-based sequencing. These emerging single-molecule protein-sequencing technologies suggests a need to develop an amino acid side chain-selective modification scheme that could target several side chains of interest. Current work for modifying residues focuses mainly on one or two side chains. The need to label many side chains, as recent computational modeling suggests, is required for high protein, sequencing coverage of the human proteome. Herein, we report our stragety for modifying two model peptides KYDWEC and KDYWE containing the most reactive residues, using highly opitmized mass labels in a sequential and selective fashion both using solution-phase and solid-phase chemistries, respectively. This will serve as a step towards a modification scheme appropriate for single-molecule studies.
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Registered trials
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