ArticleVeterinary research2017
Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis.
Article in Veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 68 citations in OpenAlex.
- Molecular epidemiological surveillance of the feline coronavirus reveals novel recombinant FCoV strains with evidence of ongoing viral evolution.Virus research · 2026Article
- A feline coronavirus nucleocapsid protein disrupts ZC3HAV1-viral RNA association to counteract host RNA-level restriction.PLoS pathogens · 2026Article
- Longitudinal detection and genetic characterization of feline coronavirus in a captive cheetah population.The Journal of general virology · 2026Article
- Multiplex fluorescent immunocytochemistry is a complementary test for the diagnosis of feline infectious peritonitis: a diagnostic clinical trial.Journal of feline medicine and surgery · 2026Article
- Development and Validation of a Triplex RT-qPCR Assay for Rapid Clinical Diagnosis and Serotyping of Feline Infectious Peritonitis Virus.International journal of molecular sciences · 2026Article
- Feline Infectious Peritonitis: A Challenging Diagnostic and Therapeutic Labyrinth.Animals : an open access journal from MDPI · 2026Review
- Review
- Rapid Clinical Resolution and Differential Diagnosis of a Neurological Case of Feline Infectious Peritonitis (FIP) Using GS-441524.Pathogens (Basel, Switzerland) · 2025Article
- Deciphering Viral Replication Dynamics in Feline Infectious Peritonitis: A Quantitative Approach.Viruses · 2025Article
- Phylogenetic, clinical, pathological and epidemiological characterization of feline coronavirus infections in cats, in Istanbul.Frontiers in veterinary science · 2025Article
- Feline Infectious Peritonitis Effusion Index: A Novel Diagnostic Method and Validation of Flow Cytometry-Based Delta Total Nucleated Cells Analysis on the Sysmex XN-1000VVeterinary sciences · 2024Article
- Evaluation of feline mesenchymal stem cell susceptibility to feline viruses.Scientific reports · 2024Article
- Clinicopathological and Molecular Analysis of Aqueous Humor for the Diagnosis of Feline Infectious Peritonitis.Veterinary sciences · 2024Article
- Article
- Retrospective study and outcome of 307 cats with feline infectious peritonitis treated with legally sourced veterinary compounded preparations of remdesivir and GS-441524 (2020-2022).Journal of feline medicine and surgery · 2023Article
- Prevalence and risk factors for common respiratory pathogens within a cohort of pet cats in the UK.The Journal of small animal practice · 2023Article
- Review
- An updated review of feline coronavirus: mind the two biotypes.Virus research · 2023Review
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic Feline Coronavirus phenotypes.bioRxiv : the preprint server for biology · 2023Article
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic feline coronavirus phenotypes.Virus evolution · 2023Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline infectious peritonitis (FIP) is a fatal disease of cats, and a sequela of systemic feline coronavirus (FCoV) infection. Mutations in the viral spike (S) gene have been associated with FCoVs found in tissues from cats with FIP, but not FCoVs found in faeces from healthy cats, and are implicated in monocyte/macrophage tropism and systemic spread. This study was designed to determine whether S gene mutation analysis can reliably diagnose FIP. Cats were categorised as with FIP (n = 57) or without FIP (n = 45) based on gross post-mortem and histopathological examination including immunohistochemistry for FCoV antigen. RNA was purified from available tissue, fluid and faeces. Reverse-transcriptase quantitative-PCR (RT-qPCR) was performed on all samples using FCoV-specific primers, followed by sequencing of a section of the S gene on RT-qPCR positive samples. Samples were available from a total of 102 cats. Tissue, fluid, and faecal samples from cats with FIP were more likely to be FCoV RT-qPCR-positive (90.4, 78.4 and 64.6% respectively) than those from cats without FIP (7.8, 2.1 and 20% respectively). Identification of S gene mutated FCoVs as an additional step to the detection of FCoV alone, only moderately increased specificity for tissue samples (from 92.6 to 94.6%) but specificity was unchanged for fluid samples (97.9%) for FIP diagnosis; however, sensitivity was markedly decreased for tissue (from 89.8 to 80.9%) and fluid samples (from 78.4 to 60%) for FIP diagnosis. These findings demonstrate that S gene mutation analysis in FCoVs does not substantially improve the ability to diagnose FIP as compared to detection of FCoV alone.
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