ArticlePediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology2017
Conditional reprogramming of pediatric airway epithelial cells: A new human model to investigate early-life respiratory disorders.
Article in Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Down Syndrome Alters Type III IFN and Proinflammatory Airway Epithelial Responses to Respiratory Syncytial Virus Infection.American journal of respiratory cell and molecular biology · 2025Article
- Human airway epithelial B-cell activating factor is reduced in early life but is virally induced via JAK/STAT.The Journal of allergy and clinical immunology · 2025Article
- High TSLP responses in the human infant airways are associated with pre-activated airway epithelial IFN antiviral immunity.Immunology · 2025Article
- Innate epithelial and functional differences in airway epithelium of children with acute wheeze.Frontiers in cell and developmental biology · 2025Article
- Impaired airway epithelial miR-155/BACH1/NRF2 axis and hypoxia gene expression during RSV infection in children with down syndrome.Frontiers in pediatrics · 2025Article
- A novel type of serum‑free medium for culturing human airway epithelium cells.Experimental and therapeutic medicine · 2023Article
- Cell-intrinsic differences between human airway epithelial cells from children and adults.iScience · 2022Article
- Conditionally reprogrammed asthmatic bronchial epithelial cells express lowerAmerican journal of physiology. Lung cellular and molecular physiology · 2022Article
- Small-molecule eRF3a degraders rescue CFTR nonsense mutations by promoting premature termination codon readthrough.The Journal of clinical investigation · 2022Article
- Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in Chronic Obstructive Pulmonary Disease.Microbiology spectrum · 2022Article
- Serially passaged, conditionally reprogrammed nasal epithelial cells as a model to study epithelial functions and SARS-CoV-2 infection.American journal of physiology. Cell physiology · 2022Article
- A medium composition containing normal resting glucose that supports differentiation of primary human airway cells.Scientific reports · 2022Article
- Long-term differentiating primary human airway epithelial cell cultures: how far are we?Cell communication and signaling : CCS · 2021Review
- Nasal Epithelial Cell-Based Models for Individualized Study in Cystic Fibrosis.International journal of molecular sciences · 2021Review
- Airway Remodeling Factors During Early-Life Rhinovirus Infection and the Effect of Premature Birth.Frontiers in pediatrics · 2021Article
- Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.bioRxiv : the preprint server for biology · 2020Article
- Conditional cell reprogramming for modeling host-virus interactions and human viral diseases.Journal of medical virology · 2020Review
- Innate IFN-lambda responses to dsRNA in the human infant airway epithelium and clinical regulatory factors during viral respiratory infections in early life.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2020Article
- Conditional reprogramming: next generation cell culture.Acta pharmaceutica Sinica. B · 2020Review
- Conditionally Reprogrammed Human Normal Airway Epithelial Cells at ALI: A Physiological Model for Emerging Viruses.Virologica Sinica · 2020Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAirway epithelial cells (AEC) are quite difficult to access in newborns and infants. It is critically important to develop robust life-extended models to conduct translational studies in this age group. We propose the use of a recently described cell culture technology (conditionally reprogrammed cells-CRC) to generate continuous primary cell cultures from nasal and bronchial AEC of young children.
methodsWe collected nasal and/or bronchial AEC from a total of 23 subjects of different ages including newborns/infants/toddlers (0-2 years; N = 9), school-age children (4-11 years; N = 6), and a group of adolescent/adult donors (N = 8). For CRC generation, we used conditioned medium from mitotically inactivated 3T3 fibroblasts and Rho-associated kinase (ROCK) inhibitor (Y-27632). Antiviral immune responses were studied using 25 key antiviral genes and protein production of type III epithelial interferon (IFN λ1) after double-stranded (ds) RNA exposure.
resultsCRC derived from primary AEC of neonates/infants and young children exhibited: (i) augmented proliferative capacity and life extension, (ii) preserved airway epithelial phenotype after multiple passages, (iii) robust immune responses characterized by the expression of innate antiviral genes and parallel nasal/bronchial production of IFN λ1 after exposure to dsRNA, and (iv) induction of airway epithelial inflammatory and remodeling responses to dsRNA (eg, CXCL8 and MMP9).
conclusionConditional reprogramming of AEC from young children is a feasible and powerful translational approach to investigate early-life airway epithelial immune responses in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.