Evidence map›Paper›PMID 28981904›Full record

ArticleProtein engineering, design & selection : PEDS2017

A panel of synthetic antibodies that selectively recognize and antagonize members of the interferon alpha family.

S Miersch, S Kuruganti, M R Walter, S S Sidhu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein engineering, design & selection : PEDS, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Antibody selection using clonal cocultivation ofProceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

S MierschThe Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6.
S KurugantiDepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
M R WalterDepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
S S SidhuThe Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6.
University of Alabama at Birmingham · USUniversity of Toronto · CA

Funding

Canadian Institutes of Health Research MOP-136944
6 · The paper itself

Abstract

The 12 distinct subtypes that comprise the interferon alpha (IFNα) family of cytokines possess anti-viral, anti-proliferative and immunomodulatory activities. They are implicated in the etiology and progression of many diseases, and also used as therapeutic agents for viral and oncologic disorders. However, a deeper understanding of their role in disease is limited by a lack of tools to evaluate single subtypes at the protein level. Antibodies that selectively inhibit single IFNα subtypes could enable interrogation of each protein in biological samples and could be used for characterization and treatment of disease. Using phage-displayed synthetic antibody libraries, we have conducted selections against 12 human IFNα subtypes to explore our ability to obtain fine-specificity antibodies that recognize and antagonize the biological signals induced by a single IFNα subtype. For the first time, we have isolated antibodies that specifically recognize individual IFNα subtypes (IFNα2a/b, IFNα6, IFNα8b and IFNα16) with high affinity that antagonize signaling. Our results show that highly specific antibodies capable of distinguishing between closely related cytokines can be isolated from synthetic libraries and can be used to characterize cytokine abundance and function.

Indexed as

Peptide LibraryAmino Acid SequenceAntibodiesAntibodies, ImmobilizedAntibody SpecificityBacteriophagesCloning, MolecularEnzyme-Linked Immunosorbent AssayEscherichia coliGene ExpressionGenes, ReporterHumansImmunoglobulin Fab FragmentsInterferon-alphaKineticsLuciferasesAntibodiesAntibodies, ImmobilizedImmunoglobulin Fab FragmentsInterferon-alphaLuciferasesPeptide LibraryProtein IsoformsRecombinant Proteinsantagonistantibody engineeringcytokineinterferon alpha interferon signalingphage display

Identifiers

PMID28981904
PMCPMC5914384
OpenAlexW2750587734

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.