ArticleGenetics and molecular biology
DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser.
Article in Genetics and molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A case of successful brood parasitism by Mandarin Duck (Aix galericulata) on Scaly-sided Merganser (Mergus squamatus).BMC zoology · 2026Article
- Disease surveillance in albatrosses and petrels from the Southwest Atlantic and Southern Ocean.Parasitology · 2025Article
- Genetic diversity and shallow genetic differentiation of the endangered scaly-sided merganserEcology and evolution · 2024Article
- Population Genomics of the Critically Endangered Brazilian Merganser.Animals : an open access journal from MDPI · 2023Article
- A novel variant of Babesia sp. (Piroplasmida) as a hemoparasite in procellariiform seabirds.Parasitology research · 2023Article
- Discovery of novel genic-SSR markers from transcriptome dataset of an important non-human primate, Macaca fascicularis.Scientific reports · 2019Article
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Authors and funding
5 authors.
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Abstract
This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a 'least concern' species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.
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