ArticlePloS one2017
A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Integrated taxonomy and genomic analysis of Marssonina zanthoxyli, the causal agent of prickly ash leaf defoliation.BMC genomics · 2026Article
- Review
- Genera of phytopathogenic fungi known from culture: 1-379.Studies in mycology · 2025Article
- Genome sequence of a EuropeanFrontiers in plant science · 2024Article
- Article
- Reducing MSH4 copy number prevents meiotic crossovers between non-homologous chromosomes in Brassica napus.Nature communications · 2019Article
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4 authors.
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Abstract
backgroundBlack spot is one of the most severe and damaging diseases of garden roses. We present the draft genome sequence of its causative agent Diplocarpon rosae as a working tool to generate molecular markers and to analyze functional and structural characteristics of this fungus.
resultsThe isolate DortE4 was sequenced with 191x coverage of different read types which were assembled into 2457 scaffolds. By evidence supported genome annotation with the MAKER pipeline 14,004 gene models were predicted and transcriptomic data indicated that 88.5% of them are expressed during the early stages of infection. Analyses of k-mer distributions resulted in unexpectedly large genome size estimations between 72.5 and 91.4 Mb, which cannot be attributed to its repeat structure and content of transposable elements alone, factors explaining such differences in other fungal genomes. In contrast, different lines of evidences demonstrate that a huge proportion (approximately 80%) of genes are duplicated, which might indicate a whole genome duplication event. By PCR-RFLP analysis of six paralogous gene pairs of BUSCO orthologs, which are expected to be single copy genes, we could show experimentally that the duplication is not due to technical error and that not all isolates tested possess all of the paralogs.
conclusionsThe presented genome sequence is still a fragmented draft but contains almost the complete gene space. Therefore, it provides a useful working tool to study the interaction of D. rosae with the host and the influence of a genome duplication outside of the model yeast in the background of a phytopathogen.
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