ArticleMicrobial biotechnology2018
Cryopreservation of artificial gut microbiota produced with in vitro fermentation technology.
Article in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 78 citations in OpenAlex.
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- Chemistry, manufacturing, and controls for live microbial products: addressing classification challenges and enhancing process validation.Frontiers in microbiomes · 2025Review
- Microbially-produced folate forms support the growth of Roseburia intestinalis but not its competitive fitness in fecal batch fermentations.BMC microbiology · 2024Article
- Trait-based study predicts glycerol/diol dehydratases as a key function of the gut microbiota of hindgut-fermenting carnivores.Microbiome · 2024Article
- The Impact of Rhizospheric and Endophytic Bacteria on the Germination ofMicroorganisms · 2024Article
- Impact of cryoprotective agents on human gut microbes and in vitro stabilized artificial gut microbiota communities.Microbial biotechnology · 2024Article
- Vitamin B12 analogues from gut microbes and diet differentially impact commensal propionate producers of the human gut.Frontiers in nutrition · 2024Article
- Fucose modifies short chain fatty acid and H2S formation through alterations of microbial cross-feeding activities.FEMS microbiology ecology · 2023Article
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- Systematic optimization of fermentation conditions forFrontiers in microbiology · 2023Article
- Review
- Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host-Microbe Interaction.Foods (Basel, Switzerland) · 2022Article
- Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples.Microbiome · 2022Article
- Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re-establishment ability.Microbial biotechnology · 2022Article
- Healthy adult gut microbiota sustains its own vitamin B12 requirement in anFrontiers in nutrition · 2022Article
- Gut Microbiota-Derived PGF2α Fights against Radiation-Induced Lung Toxicity through the MAPK/NF-κB Pathway.Antioxidants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interest in faecal microbiota transplantation (FMT) has increased as therapy for intestinal diseases, but safety issues limit its widespread use. Intestinal fermentation technology (IFT) can produce controlled, diverse and metabolically active 'artificial' colonic microbiota as potential alternative to common FMT. However, suitable processing technology to store this artificial microbiota is lacking. In this study, we evaluated the impact of the two cryoprotectives, glycerol (15% v/v) and inulin (5% w/v) alone and in combination, in preserving short-chain fatty acid formation and recovery of major butyrate-producing bacteria in three artificial microbiota during cryopreservation for 3 months at -80°C. After 24 h anaerobic fermentation of the preserved microbiota, butyrate and propionate production were maintained when glycerol was used as cryoprotectant, while acetate and butyrate were formed more rapidly with glycerol in combination with inulin. Glycerol supported cryopreservation of the Roseburia spp./Eubacterium rectale group, while inulin improved the recovery of Faecalibacterium prausnitzii. Eubacterium hallii growth was affected minimally by cryopreservation. Our data indicate that butyrate producers, which are key organisms for gut health, can be well preserved with glycerol and inulin during frozen storage. This is of high importance if artificially produced colonic microbiota is considered for therapeutic purposes.
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