ArticleFrontiers in plant science2017
An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
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- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
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- Deciphering the impact of microRNAs in plant biology: a review of computational insights and experimental validation.Molecular biology reports · 2025Review
- CRISPR-induced miRNA156-recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat.Plant biotechnology journal · 2023Article
- Recent allopolyploidy alters Spartina microRNA expression in response to xenobiotic-induced stress.Plant molecular biology · 2023Article
- Functional Analysis of Bna-miR399c-Life (Basel, Switzerland) · 2023Article
- The Ethylene Response Factor ERF5 Regulates Anthocyanin Biosynthesis in 'Zijin' Mulberry Fruits by Interacting withInternational journal of molecular sciences · 2022Article
- Integrative approaches for studying the role of noncoding RNAs in influencing drug efficacy and toxicity.Expert opinion on drug metabolism & toxicology · 2022Article
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
- A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants.Plant direct · 2020Article
- Bringing MicroRNAs to Light: Methods for MicroRNA Quantification and Visualization in Live Cells.Frontiers in bioengineering and biotechnology · 2020Review
- The Role ofInternational journal of molecular sciences · 2019Article
- Prediction and Characterization of miRNA/Target Pairs in Non-Model Plants Using RNA-seq.Current protocols in plant biology · 2019Article
- EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function inFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter genes encounter technical limitations. The aim of this study was to address the challenge of experimentally validating large numbers of computationally predicted microRNA-target transcript interactions using an optimized, quantitative, cost-effective, and scalable approach. The presented method combines transient expression via agroinfiltration of
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