ReviewFrontiers in microbiology2017
Tools for Genomic and Transcriptomic Analysis of Microbes at Single-Cell Level.
Review in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Host-gut microbiota interactions in health and disease: mechanisms and intervention strategies.Frontiers in microbiology · 2026Review
- Microbes in reconstructive restoration: Divergence in constructed and natural tree island soil fungi affects tree growth.Ecological applications : a publication of the Ecological Society of America · 2025Article
- Exploring Microbial Dark Matter for the Discovery of Novel Natural Products: Characteristics, Abundance Challenges and Methods.Journal of microbiology and biotechnology · 2024Review
- Single cell genomics based insights into the impact of cell-type specific microbial internalization on disease severity.Frontiers in immunology · 2024Review
- Single-cell analysis and spatial resolution of the gut microbiome.Frontiers in cellular and infection microbiology · 2023Review
- Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.Microorganisms · 2022Review
- One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology.Frontiers in microbiology · 2022Article
- Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels.Frontiers in microbiology · 2022Review
- Precision modification of the human gut microbiota targeting surface-associated proteins.Scientific reports · 2021Article
- Evolution of Drug-ResistantFrontiers in microbiology · 2021Review
- Microbial Single-Cell Analysis: What Can We Learn From Mammalian?Frontiers in cell and developmental biology · 2021Article
- Beyond the pan-genome: current perspectives on the functional and practical outcomes of the distributed genome hypothesis.Biochemical Society transactions · 2020Review
- Microbial single-cell omics: the crux of the matter.Applied microbiology and biotechnology · 2020Review
- Article
- Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities.Frontiers in microbiology · 2019Review
- Recent Developments in Single-Cell RNA-Seq of Microorganisms.Biophysical journal · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbiologists traditionally study population rather than individual cells, as it is generally assumed that the status of individual cells will be similar to that observed in the population. However, the recent studies have shown that the individual behavior of each single cell could be quite different from that of the whole population, suggesting the importance of extending traditional microbiology studies to single-cell level. With recent technological advances, such as flow cytometry, next-generation sequencing (NGS), and microspectroscopy, single-cell microbiology has greatly enhanced the understanding of individuality and heterogeneity of microbes in many biological systems. Notably, the application of multiple 'omics' in single-cell analysis has shed light on how individual cells perceive, respond, and adapt to the environment, how heterogeneity arises under external stress and finally determines the fate of the whole population, and how microbes survive under natural conditions. As single-cell analysis involves no axenic cultivation of target microorganism, it has also been demonstrated as a valuable tool for dissecting the microbial 'dark matter.' In this review, current state-of-the-art tools and methods for genomic and transcriptomic analysis of microbes at single-cell level were critically summarized, including single-cell isolation methods and experimental strategies of single-cell analysis with NGS. In addition, perspectives on the future trends of technology development in the field of single-cell analysis was also presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.