ArticleJournal of clinical microbiology2017
Multiplex Reverse Transcription-PCR for Simultaneous Surveillance of Influenza A and B Viruses.
Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Weak selection and stochastic processes limit the emergence of antigenic variants during household transmission of influenza A viruses.PLoS pathogens · 2026Article
- Genomic analysis of high pathogenicity avian influenza viruses from Antarctica reveals multiple introductions from South America.Nature communications · 2026Article
- Research progress on the current status of respiratory pathogen infections and their detection methods.Frontiers in microbiology · 2026Review
- Enrichment techniques for clinical metagenomics.Frontiers in cellular and infection microbiology · 2026Review
- Campus-based genomic surveillance uncovers early emergence of a future dominant A(H3N2) influenza clade.iScience · 2025Article
- Weak selection and stochastic processes limit the emergence of antigenic variants during household transmission of influenza A viruses.bioRxiv : the preprint server for biology · 2025Article
- Optimized high-throughput whole-genome sequencing workflow for surveillance of influenza A virus.Genome medicine · 2025Article
- Influenza B virus from respiratory samples of individuals with influenza-like illness in a University Campus in Arizona USA, in February 2025.Microbiology resource announcements · 2025Article
- Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR.Scientific reports · 2025Article
- Identification & genetic & virological characterisation of a human case of avian influenza A (H9N2) virus from Eastern India.The Indian journal of medical research · 2025Article
- Zanamivir and baloxavir combination to cure persistent influenza and coronavirus infections after hematopoietic stem cell transplant.International journal of antimicrobial agents · 2024Article
- A comprehensive review of influenza B virus, its biological and clinical aspects.Frontiers in microbiology · 2024Review
- Infection and tissue distribution of highly pathogenic avian influenza A type H5N1 (clade 2.3.4.4b) in red fox kits (Emerging microbes & infections · 2023Article
- Risk factors for infection with influenza A(H3N2) virus on a US university campus, October-November 2021.Influenza and other respiratory viruses · 2023Article
- Phylogenomic analysis uncovers a 9-year variation of Uganda influenza type-A strains from the WHO-recommended vaccines and other Africa strains.Scientific reports · 2023Article
- Agnostic Sequencing for Detection of Viral Pathogens.Clinical microbiology reviews · 2023Review
- Repurposing a SARS-CoV-2 surveillance program for infectious respiratory diseases in a university setting.Frontiers in public health · 2023Article
- Application value of blood metagenomic next-generation sequencing in patients with connective tissue diseases.Frontiers in immunology · 2022Article
- Whole-genome analysis to determine the rate and patterns of intra-subtype reassortment among influenza type-A viruses in Africa.Virus evolution · 2022Article
- Next-generation sequencing identification and multiplex RT-PCR detection for viruses infecting cigar and flue-cured tobacco.Molecular biology reports · 2022Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe, and influenza A viruses intermittently cause pandemics. Sequence information from influenza virus genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza virus evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral type, subtype, or lineage. Herein, we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus-positive specimens using multiple next-generation sequencing platforms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.