ArticleThe Journal of biological chemistry2017
Quantitative phosphoproteomics reveals involvement of multiple signaling pathways in early phagocytosis by the retinal pigmented epithelium.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Human Neural Progenitors Expressing GDNF Enhance Retinal Protection in a Rodent Model of Retinal Degeneration.Stem cells translational medicine · 2023Article
- Mass spectrometry-based retina proteomics.Mass spectrometry reviews · 2023Review
- qPTM: an updated database for PTM dynamics in human, mouse, rat and yeast.Nucleic acids research · 2023Article
- Phosphoproteomics data-driven signalling network inference: Does it work?Computational and structural biotechnology journal · 2023Article
- Prediction of Kinase-Substrate Associations Using The Functional Landscape of Kinases and Phosphorylation Sites.Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing · 2023Article
- Functional characterization of co-phosphorylation networks.Bioinformatics (Oxford, England) · 2022Article
- Proteins Associated with Phagocytosis Alteration in Retinal Pigment Epithelial Cells Derived from Age-Related Macular Degeneration Patients.Antioxidants (Basel, Switzerland) · 2022Article
- mTOR activity is essential for retinal pigment epithelium regeneration in zebrafish.PLoS genetics · 2022Article
- Article
- Functional Genomics of the Retina to Elucidate its Construction and Deconstruction.International journal of molecular sciences · 2019Review
- Rev-Erbα and Photoreceptor Outer Segments modulate the Circadian Clock in Retinal Pigment Epithelial Cells.Scientific reports · 2019Article
- Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.Cell reports · 2019Article
- Rethinking Phagocytes: Clues from the Retina and Testes.Trends in cell biology · 2018Review
- Comprehensive characterization of the adult ND4 Swiss Webster mouse retina: Using discovery-based mass spectrometry to decipher the total proteome and phosphoproteome.Molecular vision · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 5 countries.
Funding
Abstract
One of the major biological functions of the retinal pigmented epithelium (RPE) is the clearance of shed photoreceptor outer segments (POS) through a multistep process resembling phagocytosis. RPE phagocytosis helps maintain the viability of photoreceptors that otherwise could succumb to the high metabolic flux and photo-oxidative stress associated with visual processing. The regulatory mechanisms underlying phagocytosis in the RPE are not fully understood, although dysfunction of this process contributes to the pathogenesis of multiple human retinal degenerative disorders, including age-related macular degeneration. Here, we present an integrated transcriptomic, proteomic, and phosphoproteomic analysis of phagocytosing RPE cells, utilizing three different experimental models: the human-derived RPE-like cell line ARPE-19, cultured murine primary RPE cells, and RPE samples from live mice. Our combined results indicated that early stages of phagocytosis in the RPE are mainly characterized by pronounced changes in the protein phosphorylation level. Global phosphoprotein enrichment analysis revealed involvement of PI3K/Akt, mechanistic target of rapamycin (mTOR), and MEK/ERK pathways in the regulation of RPE phagocytosis, confirmed by immunoblot analyses and
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Registered trials
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