ArticleAmerican journal of physiology. Renal physiology2018
NMR spectroscopy and electron microscopy identification of metabolic and ultrastructural changes to the kidney following ischemia-reperfusion injury.
Article in American journal of physiology. Renal physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.
- Exosome Biogenesis: Meta-Analysis of Intraluminal Vesicle Size Across Species.International journal of molecular sciences · 2026Pooled it
- Preclinical models versus clinical renal ischemia reperfusion injury: A systematic review based on metabolic signatures.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Pooled it
- Understanding the Metabolic Effects of Surgically Induced Renal Ischemia in Humans: A Temporal Approach.Metabolites · 2025Article
- Article
- Renal microcirculation and mechanisms in diabetic kidney disease.Frontiers in endocrinology · 2025Review
- Metabolomic and transcriptomic insights into the mechanisms of renal ischemia-reperfusion injury progression.Scientific reports · 2024Article
- ATP dynamics as a predictor of future podocyte structure and function after acute ischemic kidney injury in female mice.Nature communications · 2024Article
- Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis.Nature communications · 2024Article
- Statistical considerations and database limitations in NMR-based metabolic profiling studies.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- Review
- Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice.Nature communications · 2023Article
- Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.Experimental animals · 2022Article
- A Review of Current and Emerging Trends in Donor Graft-Quality Assessment Techniques.Journal of clinical medicine · 2022Review
- Targeting the innate repair receptor axisFrontiers in immunology · 2022Article
- NMR-based serum and urine metabolomic profile reveals suppression of mitochondrial pathways in experimental sepsis-associated acute kidney injury.American journal of physiology. Renal physiology · 2021Article
- Effects of Ischemia-Reperfusion on Tubular Cell Membrane Transporters and Consequences in Kidney Transplantation.Journal of clinical medicine · 2020Review
- Urinary NMR Profiling in Pediatric Acute Kidney Injury-A Pilot Study.International journal of molecular sciences · 2020Article
- The Current State of the Art in Acute Kidney Injury.Frontiers in pediatrics · 2020Review
- Article
- Novel kidney dissociation protocol and image-based flow cytometry facilitate improved analysis of injured proximal tubules.American journal of physiology. Renal physiology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cellular, molecular, and ultrastructural nephron changes associated with ischemia-reperfusion injury-induced acute kidney injury (IRI-AKI) are not completely understood. Here, a multidisciplinary study was used to identify nephron changes in a mouse model of IRI-AKI. Histological analyses indicated distended Bowman's glomerular spaces and proximal and distal tubules. Increased filtrate volume in nephrons was caused by reduced water reabsorption by severely damaged proximal tubule brush borders and blocked flow of filtrate into collecting tubules by mucoprotein casts in distal tubules. Immunohistochemistry revealed protein AKI biomarkers in proximal tubules and glomeruli but not in distal tubules. Nuclear magnetic resonance spectroscopy revealed several metabolites that increased such as valine, alanine, and lactate. Other metabolites such as trigonelline, succinate, 2-oxoisocaproate, and 1- methyl-nicotinamide decreased or were absent in urine following IRI due to altered kidney function or metabolism. Urinary glucose increased due to reduced reabsorption by damaged proximal tubule brush borders. Scanning electron microscopy revealed flattening of podocytes and pedicals surrounding glomerular capillaries, and transmission electron microscopy (TEM) revealed effacement of podocyte pedicals, both consistent with increased hydrostatic pressure in nephrons following IRI-AKI. TEM revealed shortened proximal tubule microvilli in IRI kidneys with diminished lamina propia. TEM showed dramatic loss of mitochondria in distal tubule epithelia of IRI kidneys and emergence of multivesicular bodies of endosomes indicating ongoing cellular death. Collectively, the data define ultrastructural changes to nephrons and altered kidney metabolism associated with IRI-AKI.
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Registered trials
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