Evidence map›Paper›PMID 28978449›Full record

ArticleBiophysical journal2017

Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties.

Kendra D Nyberg, Kenneth H Hu, Sara H Kleinman, Damir B Khismatullin, Manish J Butte, Amy C Rowat

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Review
  2. Article
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  5. Article
  6. A microfluidic rheometer for tumor mechanics and invasion studies.bioRxiv : the preprint server for biology · 2025
    Article
  7. Article
  8. Characterization of cellular wound resistance in the giant ciliatebioRxiv : the preprint server for biology · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025
    Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kendra D NybergDepartment of Integrative Biology and Physiology, University of California, Los Angeles, California; Department of Bioengineering, University of California, Los Angeles, California.
Kenneth H HuDepartment of Physics, Stanford University, Stanford, California.
Sara H KleinmanDepartment of Pediatrics, Stanford University, Stanford, California.
Damir B KhismatullinDepartment of Biomedical Engineering, Tulane University, New Orleans, Louisiana.
Manish J ButteDepartment of Pediatrics, University of California, Los Angeles, California; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California.
Amy C RowatDepartment of Integrative Biology and Physiology, University of California, Los Angeles, California; Department of Bioengineering, University of California, Los Angeles, California; UCLA Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California; Broad Stem Cell Research Center, University of California, Los Angeles, California; Center for Biological Physics, University of California, Los Angeles, California. Electronic address: rowat@ucla.edu.

Funding

Molecular Biophysics Training Program at StanfordT32GM008294 · NIGMS · STANFORD UNIVERSITY · PI HUANG, KERWYN C. · 1989 to 2019
$7.4M
Influences of nanomechanical forces on T cellsR01GM110482 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUTTE, MANISH J · 2014 to 2018
$1.6M
NIGMS NIH HHS R01 GM110482NIGMS NIH HHS T32 GM008294
6 · The paper itself

Abstract

Advances in methods that determine cell mechanical phenotype, or mechanotype, have demonstrated the utility of biophysical markers in clinical and research applications ranging from cancer diagnosis to stem cell enrichment. Here, we introduce quantitative deformability cytometry (q-DC), a method for rapid, calibrated, single-cell mechanotyping. We track changes in cell shape as cells deform into microfluidic constrictions, and we calibrate the mechanical stresses using gel beads. We observe that time-dependent strain follows power-law rheology, enabling single-cell measurements of apparent elastic modulus, E

Indexed as

Cell Physiological PhenomenaMicrofluidic Analytical TechniquesSingle-Cell AnalysisBiomechanical PhenomenaCalibrationCell Line, TumorCell ShapeComputer SimulationCytoskeletonElastic ModulusEquipment DesignHumansLab-On-A-Chip DevicesModels, BiologicalSepharoseSilicone OilsSepharoseSilicone Oils

Identifiers

PMID28978449
PMCPMC5627151

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.