ArticleNucleic acids research2017
Programming sites of meiotic crossovers using Spo11 fusion proteins.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Optimum recombination rates for genetic gains in simulated recurrent selection in empirical maize populations.The plant genome · 2026Article
- Chromosomal structural variation loci HSS1 and HSS6 lead to hybrid sterility in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- The future of genome editing in plants.Nature plants · 2025Review
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Crop bioengineering via gene editing: reshaping the future of agriculture.Plant cell reports · 2024Review
- Efficient targeted recombination with CRISPR/Cas9 in hybrids of Caenorhabditis nematodes with suppressed recombination.BMC biology · 2023Article
- Dual roles of R-loops in the formation and processing of programmed DNA double-strand breaks during meiosis.Cell & bioscience · 2023Article
- Exploring impact of recombination landscapes on breeding outcomes.Proceedings of the National Academy of Sciences of the United States of America · 2023Review
- Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants.Plant biotechnology journal · 2023Article
- Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis.G3 (Bethesda, Md.) · 2022Article
- Review
- Genomic prediction of the recombination rate variation in barley - A route to highly recombinogenic genotypes.Plant biotechnology journal · 2022Article
- Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering.Plant physiology · 2022Article
- Review
- Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair.Nucleic acids research · 2022Article
- Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022Review
- Genetic studies of human-chimpanzee divergence using stem cell fusions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Attaining the promise of plant gene editing at scale.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- CRISPR-Cas-mediated chromosome engineering for crop improvement and synthetic biology.Nature plants · 2021Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Meiotic recombination shapes the genetic diversity transmitted upon sexual reproduction. However, its non-random distribution along the chromosomes constrains the landscape of potential genetic combinations. For a variety of purposes, it is desirable to expand the natural repertoire by changing the distribution of crossovers in a wide range of eukaryotes. Toward this end, we report the local stimulation of meiotic recombination at a number of chromosomal sites by tethering the natural Spo11 protein to various DNA-binding modules: full-length DNA binding proteins, zinc fingers (ZFs), transcription activator-like effector (TALE) modules, and the CRISPR-Cas9 system. In the yeast Saccharomyces cerevisiae, each strategy is able to stimulate crossover frequencies in naturally recombination-cold regions. The binding and cleavage efficiency of the targeting Spo11 fusions (TSF) are variable, being dependent on the chromosomal regions and potential competition with endogenous factors. TSF-mediated genome interrogation distinguishes naturally recombination-cold regions that are flexible and can be warmed-up (gene promoters and coding sequences), from those that remain refractory (gene terminators and centromeres). These results describe new generic experimental strategies to increase the genetic diversity of gametes, which should prove useful in plant breeding and other applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.