ArticleNucleic acids research2017
In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 23 citations in OpenAlex.
- Active-learning-guided optimization of cell-free systems for genome-wide transcriptomic profiling reveals progressive layers of regulation.Nature communications · 2026Article
- Chaperone dependency during biogenesis does not correlate with chaperone dependency during refolding.Molecular systems biology · 2026Article
- Bacterial chromosome conformation and cell-free gene expression in synthetic 2D compartments.Nature communications · 2025Article
- Chaperone Dependency during Primary Protein Biogenesis Does Not Correlate with Chaperone Dependency duringbioRxiv : the preprint server for biology · 2025Article
- Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.Molecules (Basel, Switzerland) · 2024Article
- Leveraging Active Learning to Establish Efficient In Vitro Transcription and Translation from Bacterial Chromosomal DNA.ACS omega · 2024Article
- Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium.ACS synthetic biology · 2023Article
- Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides.Frontiers in bioengineering and biotechnology · 2022Review
- A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression.Life (Basel, Switzerland) · 2021Article
- Article
- Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions.ACS synthetic biology · 2020Article
- Metabolic Profiling ofIndustrial & engineering chemistry research · 2019Article
- A Modular Vector Toolkit with a Tailored Set of Thermosensors To Regulate Gene Expression inACS omega · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In vitro transcription-translation systems (TX-TL) can synthesize most of individual genes encoded in genomes by using strong promoters and translation initiation sequences. This fact raises a possibility that TX-TL using genome as a template can reconstitute the profile of RNA and proteins in living cells. By using cell extracts and genome prepared from different organisms, here we developed a system for in vitro genome transcription-translation (iGeTT) using bacterial genome and cell extracts, and surveyed de novo synthesis of RNA and proteins. Two-dimensional electrophoresis and nano LC-MS/MS showed that proteins were actually expressed by iGeTT. Quantitation of transcription levels of 50 genes for intracellular homeostasis revealed that the levels of RNA synthesis by iGeTT are highly correlated with those in growth phase cells. Furthermore, activity of iGeTT was influenced by transcription derived from genome structure and gene location in genome. These results suggest that intracellular profiles and characters of genome can be emulated by TX-TL using genome as a template.
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