ArticleNucleic acids research2018
qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed.
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- Analysis of the Mechanism of Wood Vinegar and Butyrolactone Promoting Rapeseed Growth and Improving Low-Temperature Stress Resistance Based on Transcriptome and Metabolomics.International journal of molecular sciences · 2024Article
- Exploring silique number in Brassica napus L.: Genetic and molecular advances for improving yield.Plant biotechnology journal · 2024Review
- GhRCD1 promotes cotton tolerance to cadmium by regulating the GhbHLH12-GhMYB44-GhHMA1 transcriptional cascade.Plant biotechnology journal · 2024Article
- TheInternational journal of molecular sciences · 2024Article
- Heterologous overexpression of heat shock protein 20 genes of different species of yellow Camellia in Arabidopsis thaliana reveals their roles in high calcium resistance.BMC plant biology · 2024Article
- Regulation of micro- and small-exon retention and other splicing processes by GRP20 for flower development.Nature plants · 2024Article
- Comprehensive identification and functional characterization of GhpPLA gene family in reproductive organ development.BMC plant biology · 2023Article
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- Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics.Metabolites · 2023Article
- Identification of a Rice Leaf Width GeneInternational journal of molecular sciences · 2023Article
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
16 more citing papers are in PubMed but not listed here.
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21 authors.
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Abstract
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerDB database, founded on an automatic gene-specific qPCR primer design and thermodynamics-based validation workflow. The qPrimerDB database is the most comprehensive qPCR primer database available to date, with a web front-end providing gene-specific and pre-computed primer pairs across 147 important organisms, including human, mouse, zebrafish, yeast, thale cress, rice and maize. In this database, we provide 3331426 of the best primer pairs for each gene, based on primer pair coverage, as well as 47760359 alternative gene-specific primer pairs, which can be conveniently batch downloaded. The specificity and efficiency was validated for qPCR primer pairs for 66 randomly selected genes, in six different organisms, through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis. This resource, which will be routinely updated, is publically accessible at http://biodb.swu.edu.cn/qprimerdb.
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