ArticlePLoS pathogens2017
Rice stripe virus NS3 protein regulates primary miRNA processing through association with the miRNA biogenesis factor OsDRB1 and facilitates virus infection in rice.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Tobacco rattle virus infection alters Arabidopsis gene expression via virus-derived SiRNAs and host MiRNA network disruption.BMC genomics · 2025Article
- A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis.Nature communications · 2025Article
- Article
- UBL5 and Its Role in Viral Infections.Viruses · 2024Review
- Citrus psorosis virus 24K protein inhibits the processing of miRNA precursors by interacting with components of the biogenesis machinery.Microbiology spectrum · 2024Article
- Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking.Plant biotechnology journal · 2024Article
- Polyubiquitylated rice stripe virus NS3 translocates to the nucleus to promote cytosolic virus replication via miRNA-induced fibrillin 2 upregulation.PLoS pathogens · 2024Article
- Rice stripe virus nonstructural protein 3 suppresses plant defence responses mediated by the MEL-SHMT1 module.Molecular plant pathology · 2023Article
- Sugarcane streak mosaic virus P1 protein inhibits unfolded protein response through direct suppression of bZIP60U splicing.PLoS pathogens · 2023Article
- A Novel miRNA in Rice Associated with the Low Seed Setting Rate Symptom of Rice Stripe Virus.International journal of molecular sciences · 2023Article
- Silencing ofPlant signaling & behavior · 2022Article
- A Review of Vector-Borne Rice Viruses.Viruses · 2022Review
- Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions.Stress biology · 2022Review
- The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication.Protein & cell · 2022Article
- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Global Identification and Characterization of C2 Domain-Containing Proteins Associated with Abiotic Stress Response in Rice (International journal of molecular sciences · 2022Article
- Insights Into the Effect of Rice Stripe Virus P2 on Rice Defense by Comparative Proteomic Analysis.Frontiers in microbiology · 2022Article
- P25 and P37 proteins encoded by firespike leafroll-associated virus are viral suppressors of RNA silencing.Frontiers in microbiology · 2022Article
- Article
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small regulatory RNAs processed from primary miRNA transcripts, and plant miRNAs play important roles in plant growth, development, and response to infection by microbes. Microbial infections broadly alter miRNA biogenesis, but the underlying mechanisms remain poorly understood. In this study, we report that the Rice stripe virus (RSV)-encoded nonstructural protein 3 (NS3) interacts with OsDRB1, an indispensable component of the rice (Oryza sativa) miRNA-processing complex. Moreover, the NS3-OsDRB1 interaction occurs at the sites required for OsDRB1 self-interaction, which is essential for miRNA biogenesis. Further analysis revealed that NS3 acts as a scaffold between OsDRB1 and pri-miRNAs to regulate their association and aids in vivo processing of pri-miRNAs. Genetic evidence in Arabidopsis showed that NS3 can partially substitute for the function of double-stranded RNA binding domain (dsRBD) of AtDRB1/AtHYL1 during miRNA biogenesis. As a result, NS3 induces the accumulation of several miRNAs, most of which target pivotal genes associated with development or pathogen resistance. In contrast, a mutant version of NS3 (mNS3), which still associated with OsDRB1 but has defects in pri-miRNA binding, reduces accumulation of these miRNAs. Transgenic rice lines expressing NS3 exhibited significantly higher susceptibility to RSV infection compared with non-transgenic wild-type plants, whereas the transgenic lines expressing mNS3 showed a less-sensitive response. Our findings revealed a previously unknown mechanism in which a viral protein hijacks OsDRB1, a key component of the processing complex, for miRNA biogenesis and enhances viral infection and pathogenesis in rice.
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