ArticleBiochemistry2017
Purification and Characterization of RhoPDE, a Retinylidene/Phosphodiesterase Fusion Protein and Potential Optogenetic Tool from the Choanoflagellate Salpingoeca rosetta.
Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025Review
- Characterization and Modification of Light-Sensitive Phosphodiesterases from Choanoflagellates.Biomolecules · 2022Article
- From the Sea for the Sight: Marine Derived Products for Human Vision.Frontiers in aging neuroscience · 2022Review
- Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular Biology of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Article
- Advances and prospects of rhodopsin-based optogenetics in plant research.Plant physiology · 2021Review
- Molecular Properties and Optogenetic Applications of Enzymerhodopsins.Advances in experimental medicine and biology · 2021Review
- Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases.Frontiers in molecular biosciences · 2021Review
- Structural insights into the mechanism of rhodopsin phosphodiesterase.Nature communications · 2020Article
- Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems.Life (Basel, Switzerland) · 2020Article
- Advances, Perspectives and Potential Engineering Strategies of Light-Gated Phosphodiesterases for Optogenetic Applications.International journal of molecular sciences · 2020Review
- Article
- A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Therapeutic targeting of 3',5'-cyclic nucleotide phosphodiesterases: inhibition and beyond.Nature reviews. Drug discovery · 2019Review
- Spectroscopic study of the transmembrane domain of a rhodopsin-phosphodiesterase fusion protein from a unicellular eukaryote.The Journal of biological chemistry · 2019Article
- Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.BMC biology · 2018Article
- Structure and monomer/dimer equilibrium for the guanylyl cyclase domain of the optogenetics protein RhoGC.The Journal of biological chemistry · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
RhoPDE is a type I rhodopsin/phosphodiesterase gene fusion product from the choanoflagellate Salpingoeca rosetta. The gene was discovered around the time that a similar type I rhodopsin/guanylyl cyclase fusion protein, RhoGC, was shown to control phototaxis of an aquatic fungus through a cGMP signaling pathway. RhoPDE has potential as an optogenetic tool catalyzing the hydrolysis of cyclic nucleotides. Here we provide an expression and purification system for RhoPDE, as well as a crystal structure of the C-terminal phosphodiesterase catalytic domain. We show that RhoPDE contains an even number of transmembrane segments, with N- and C-termini both located on the cytoplasmic surface of the cell membrane. The purified protein exhibits an absorption maximum at 490 nm in the dark state, which shifts to 380 nm upon exposure to light. The protein acts as a cGMP-selective phosphodiesterase. However, the activity does not appear to be modulated by light. The protein is also active with cAMP as a substrate, but with a roughly 5-7-fold lower k
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.