ArticleScience advances2017
Feedback-tracking microrheology in living cells.
Article in Science advances, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 79 citations in OpenAlex.
- Real-Time Monitoring of Mass and Mechanical Properties in Single Cells and Multicellular Spheroids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospring.eLife · 2026Article
- An active matter model captures spatial dynamics of actomyosin oscillations in larval epithelial cells duringProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Bidirectional quantitative scattering microscopy.Nature communications · 2025Article
- Microrheology: From Video Microscopy to Optical Tweezers.Micromachines · 2025Review
- Advanced Microarrays as Heterogeneous Force-Remodeling Coordinator to Orchestrate Nuclear Configuration and Force-Sensing Mechanotransduction in Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Mechanical Forces, Nucleus, Chromosomes, and Chromatin.Biomolecules · 2025Review
- Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology.Nature nanotechnology · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Rapid mechanical phenotyping of breast cancer cells based on stochastic intracellular fluctuations.iScience · 2024Article
- Nonthermal fluctuations accelerate biomolecular motors.Biophysical reviews · 2024Review
- Cytoplasmic fluidization contributes to breaking spore dormancy in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Activity-dependent glassy cell mechanics II: Nonthermal fluctuations under metabolic activity.Biophysical journal · 2023Article
- Simultaneous Nanorheometry and Nanothermometry Using Intracellular Diamond Quantum Sensors.ACS nano · 2023Article
- Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology.Biophysical journal · 2023Article
- Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes:Interface focus · 2022Article
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Nonlinear Elastic and Inelastic Properties of Cells.Journal of biomechanical engineering · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Living cells are composed of active materials, in which forces are generated by the energy derived from metabolism. Forces and structures self-organize to shape the cell and drive its dynamic functions. Understanding the out-of-equilibrium mechanics is challenging because constituent materials, the cytoskeleton and the cytosol, are extraordinarily heterogeneous, and their physical properties are strongly affected by the internally generated forces. We have analyzed dynamics inside two types of eukaryotic cells, fibroblasts and epithelial-like HeLa cells, with simultaneous active and passive microrheology using laser interferometry and optical trapping technology. We developed a method to track microscopic probes stably in cells in the presence of vigorous cytoplasmic fluctuations, by using smooth three-dimensional (3D) feedback of a piezo-actuated sample stage. To interpret the data, we present a theory that adapts the fluctuation-dissipation theorem (FDT) to out-of-equilibrium systems that are subjected to positional feedback, which introduces an additional nonequilibrium effect. We discuss the interplay between material properties and nonthermal force fluctuations in the living cells that we quantify through the violations of the FDT. In adherent fibroblasts, we observed a well-known polymer network viscoelastic response where the complex shear modulus scales as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.