Evidence map›Paper›PMID 28974747›Full record

ArticleScientific reports2017

Annexin-enriched osteoblast-derived vesicles act as an extracellular site of mineral nucleation within developing stem cell cultures.

O G Davies, S C Cox, R L Williams, D Tsaroucha, R M Dorrepaal, M P Lewis, L M Grover

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Extracellular vesicles: From bone development to regenerative orthopedics.Molecular therapy : the journal of the American Society of Gene Therapy · 2023
    Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

O G DaviesSchool of Sport, Exercise and Health Sciences, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK. O.G.Davies@lboro.ac.uk.
S C CoxSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
R L WilliamsSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
D TsarouchaSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
R M DorrepaalUCD School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin, 4, Ireland.
M P LewisSchool of Sport, Exercise and Health Sciences, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK.
L M GroverSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

Funding

Medical Research Council MC_PC_16037
6 · The paper itself

Abstract

The application of extracellular vesicles (EVs) as natural delivery vehicles capable of enhancing tissue regeneration could represent an exciting new phase in medicine. We sought to define the capacity of EVs derived from mineralising osteoblasts (MO-EVs) to induce mineralisation in mesenchymal stem cell (MSC) cultures and delineate the underlying biochemical mechanisms involved. Strikingly, we show that the addition of MO-EVs to MSC cultures significantly (P < 0.05) enhanced the expression of alkaline phosphatase, as well as the rate and volume of mineralisation beyond the current gold-standard, BMP-2. Intriguingly, these effects were only observed in the presence of an exogenous phosphate source. EVs derived from non-mineralising osteoblasts (NMO-EVs) were not found to enhance mineralisation beyond the control. Comparative label-free LC-MS/MS profiling of EVs indicated that enhanced mineralisation could be attributed to the delivery of bridging collagens, primarily associated with osteoblast communication, and other non-collagenous proteins to the developing extracellular matrix. In particular, EV-associated annexin calcium channelling proteins, which form a nucleational core with the phospholipid-rich membrane and support the formation of a pre-apatitic mineral phase, which was identified using infrared spectroscopy. These findings support the role of EVs as early sites of mineral nucleation and demonstrate their value for promoting hard tissue regeneration.

Indexed as

Alkaline PhosphataseAnnexinsBone Morphogenetic Protein 2Cell Culture TechniquesCell DifferentiationChromatography, LiquidExtracellular MatrixExtracellular VesiclesHumansMesenchymal Stem CellsMesenchymal Stem Cell TransplantationOsteoblastsRegenerationTandem Mass SpectrometryAlkaline PhosphataseAnnexinsBMP2 protein, humanBone Morphogenetic Protein 2

Identifiers

PMID28974747
PMCPMC5626761

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.