ArticleScientific reports2017
Role of autophagy in cell-penetrating peptide transfection model.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
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- Antimicrobial peptides with cell-penetrating activity as prophylactic and treatment drugs.Bioscience reports · 2022Review
- A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence.Nature biomedical engineering · 2022Article
- Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes.Cells · 2022Article
- Improvement of Transfection with PepFects Using Organic and Inorganic Materials.Methods in molecular biology (Clifton, N.J.) · 2022Article
- PepFect14 Signaling and Transfection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Approaches for evaluation of novel CPP-based cargo delivery systems.Frontiers in pharmacology · 2022Review
- Cell-Penetrating Peptides and Transportan.Pharmaceutics · 2021Review
- HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner.Autophagy · 2021Article
- Antimicrobial Peptide againstPharmaceutics · 2020Article
- Article
- A novel and translational role for autophagy in antisense oligonucleotide trafficking and activity.Nucleic acids research · 2019Article
- Cell-Penetrating Peptides to Enhance Delivery of Oligonucleotide-Based Therapeutics.Biomedicines · 2018Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-penetrating peptides (CPPs) uptake mechanism is still in need of more clarification to have a better understanding of their action in the mediation of oligonucleotide transfection. In this study, the effect on early events (1 h treatment) in transfection by PepFect14 (PF14), with or without oligonucleotide cargo on gene expression, in HeLa cells, have been investigated. The RNA expression profile was characterized by RNA sequencing and confirmed by qPCR analysis. The gene regulations were then related to the biological processes by the study of signaling pathways that showed the induction of autophagy-related genes in early transfection. A ligand library interfering with the detected intracellular pathways showed concentration-dependent effects on the transfection efficiency of splice correction oligonucleotide complexed with PepFect14, proving that the autophagy process is induced upon the uptake of complexes. Finally, the autophagy induction and colocalization with autophagosomes have been confirmed by confocal microscopy and transmission electron microscopy. We conclude that autophagy, an inherent cellular response process, is triggered by the cellular uptake of CPP-based transfection system. This finding opens novel possibilities to use autophagy modifiers in future gene therapy.
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