Evidence map›Paper›PMID 28974693›Full record

ArticleNature microbiology2017

Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation.

Erkin Kuru, Carey Lambert, Jonathan Rittichier, Rob Till, Adrien Ducret, Adeline Derouaux, Joe Gray, Jacob Biboy, Waldemar Vollmer, Michael VanNieuwenhze and 2 more

Erratum issuedOpen access · greenAbstract read
In one paragraph

Article in Nature microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
7.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 128 citations in OpenAlex.

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  18. Omics for deciphering oral microecology.International journal of oral science · 2024
    Review
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 4 countries.

Erkin KuruMolecular and Cellular Biochemistry Department, Indiana University Bloomington, Bloomington, IN, 47405, USA.
Carey LambertDepartment of Biology, Indiana University Bloomington, Bloomington, IN, 47405, USA.
Jonathan RittichierSchool of Life Sciences, Nottingham University, Queen's Medical Centre, Nottingham, NG7 2UH, UK.
Rob TillDepartment of Biology, Indiana University Bloomington, Bloomington, IN, 47405, USA.
Adrien DucretDepartment of Chemistry, Indiana University Bloomington, Bloomington, IN, 47405, USA.
Adeline DerouauxThe Centre for Bacterial Cell Biology, Baddiley Clark Building, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.
Joe GrayThe Centre for Bacterial Cell Biology, Baddiley Clark Building, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.
Jacob BiboyThe Centre for Bacterial Cell Biology, Baddiley Clark Building, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.
Waldemar VollmerThe Centre for Bacterial Cell Biology, Baddiley Clark Building, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne, NE2 4AX, UK.
Michael VanNieuwenhzeSchool of Life Sciences, Nottingham University, Queen's Medical Centre, Nottingham, NG7 2UH, UK.
Yves V BrunDepartment of Chemistry, Indiana University Bloomington, Bloomington, IN, 47405, USA.
R Elizabeth SockettDepartment of Biology, Indiana University Bloomington, Bloomington, IN, 47405, USA. liz.sockett@nottingham.ac.uk.ORCID http://orcid.org/0000-0002-6271-2674
Indiana University Bloomington · USNewcastle University · GBHarvard University · USQueen's Medical Centre · GBUniversité Claude Bernard Lyon 1 · FRUniversity of Liège · BE

Funding

GLOBAL CONTROL OF DIFFERENTIATION IN CAULOBACTERR01GM051986 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI BRUN, YVES V · 1995 to 2016
$6.0M
Dynamics of bacterial peptidoglycan synthesisR01GM113172 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI BRUN, YVES V, VANNIEUWENHZE, MICHAEL S · 2015 to 2018
$3.5M
Bacterial Subcellular Organization and its Impact on Growth, Development, Aging, and Surface AdhesionR35GM122556 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI BRUN, YVES V · 2017 to 2019
$1.6M
Medical Research Council MR/K015753/1NIGMS NIH HHS R01 GM051986NIGMS NIH HHS R01 GM113172NIGMS NIH HHS R35 GM122556Wellcome Trust
6 · The paper itself

Abstract

Modification of essential bacterial peptidoglycan (PG)-containing cell walls can lead to antibiotic resistance; for example, β-lactam resistance by L,D-transpeptidase activities. Predatory Bdellovibrio bacteriovorus are naturally antibacterial and combat infections by traversing, modifying and finally destroying walls of Gram-negative prey bacteria, modifying their own PG as they grow inside prey. Historically, these multi-enzymatic processes on two similar PG walls have proved challenging to elucidate. Here, with a PG-labelling approach utilizing timed pulses of multiple fluorescent D-amino acids, we illuminate dynamic changes that predator and prey walls go through during the different phases of bacteria:bacteria invasion. We show formation of a reinforced circular port-hole in the prey wall, L,D-transpeptidase

Indexed as

Amino AcidsAmino Acids, DiaminoBacterial ProteinsBdellovibrioBdellovibrio bacteriovorusCell WallEscherichia coliGenes, BacterialGram-Negative BacteriaPeptidoglycanPeptidyl TransferasesSequence DeletionTime FactorsAmino AcidsAmino Acids, DiaminoBacterial ProteinsPeptidoglycanPeptidyl Transferases

Identifiers

PMID28974693
PMCPMC5705579
OpenAlexW2763716316

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.