ArticleMalaria journal2017
Species and genotype diversity of Plasmodium in malaria patients from Gabon analysed by next generation sequencing.
Article in Malaria journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Over-dispersion in malaria-schistosoma co-infection: insights from a meta-analytical approach and systematic review.Infectious diseases of poverty · 2026Pooled it
- Association of interleukin-33 gene polymorphisms with susceptibility to Plasmodium falciparum infection.Malaria journal · 2026Article
- Assessment of automated loop-mediated isothermal amplification-(LAMP-)based xenomonitoring for Plasmodium spp. in Anopheles mosquitoes.Malaria journal · 2026Article
- Mobile phone-based plasmodium parasites stage detection from Giemsa stained blood smear by convolutional neural networks.Parasitology research · 2025Article
- Indole-2-carboxamides Optimization for Antiplasmodial Activity.ACS bio & med chem Au · 2025Article
- Prevalence of resistance markers of artemisinin, partner drugs, and sulfadoxine-pyrimethamine in Nanyumbu and Masasi Districts, Tanzania between 2020 and 2021.Antimicrobial agents and chemotherapy · 2025Article
- Reduction in Asymptomatic falciparum malaria infection amongst schoolchildren in three ecological zones of Ghana between 2017 and 2023.Scientific reports · 2025Article
- Mass spectrometry-based metabolomics uncovers distinct metabolic signatures and potential therapeutic targets in Plasmodium knowlesi.PloS one · 2025Article
- Epidemiology and diversity of Plasmodium species in Franceville and their implications for malaria control.Scientific reports · 2024Article
- Evidence of Plasmodium vivax circulation in western and eastern regions of Senegal: implications for malaria control.Malaria journal · 2024Article
- Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing.Malaria journal · 2024Article
- Genetic diversity ofFrontiers in cellular and infection microbiology · 2024Article
- Assessment of genetic polymorphisms associated with malaria antifolate resistance among the population of Libreville, Gabon.Malaria journal · 2023Article
- Decrease on malaria clinical cases from 2017 to 2019 in Franceville, Southeast Gabon, Central Africa.Journal of public health in Africa · 2023Article
- CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification.Frontiers in microbiology · 2023Review
- Malaria: An Overview.Infection and drug resistance · 2023Review
- Codependence of individuals in the Nephromyces species swarm requires heterospecific bacterial endosymbionts.Current biology : CB · 2022Article
- Systematic Review on Diversity and Distribution ofPathogens (Basel, Switzerland) · 2022Review
- Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria.Biology · 2022Review
- An analysis ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSix Plasmodium species are known to naturally infect humans. Mixed species infections occur regularly but morphological discrimination by microscopy is difficult and multiplicity of infection (MOI) can only be evaluated by molecular methods. This study investigated the complexity of Plasmodium infections in patients treated for microscopically detected non-falciparum or mixed species malaria in Gabon.
methodsUltra-deep sequencing of nucleus (18S rRNA), mitochondrion, and apicoplast encoded genes was used to evaluate Plasmodium species diversity and MOI in 46 symptomatic Gabonese patients with microscopically diagnosed non-falciparum or mixed species malaria.
resultsDeep sequencing revealed a large complexity of confections in patients with uncomplicated malaria, both on species and genotype levels. Mixed infections involved up to four parasite species (Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale curtisi, and P. ovale wallikeri). Multiple genotypes from each species were determined from the asexual 18S rRNA gene. 17 of 46 samples (37%) harboured multiple genotypes of at least one Plasmodium species. The number of genotypes per sample (MOI) was highest in P. malariae (n = 4), followed by P. ovale curtisi (n = 3), P. ovale wallikeri (n = 3), and P. falciparum (n = 2). The highest combined genotype complexity in samples that contained mixed-species infections was seven.
conclusionsUltra-deep sequencing showed an unexpected breadth of Plasmodium species and within species diversity in clinical samples. MOI of P. ovale curtisi, P. ovale wallikeri and P. malariae infections were higher than anticipated and contribute significantly to the burden of malaria in Gabon.
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