ReviewMicrobial cell factories2017
Methods for genetic transformation of filamentous fungi.
Review in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed, 1 synthesis or guideline pooled it, 203 citations in OpenAlex.
- BioprospectingFrontiers in fungal biology · 2021Pooled it
- Barcoded mutant library enables high-throughput functional genomics in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Establishment of Protoplast Preparation and Genetic Transformation Methods in TwoJournal of fungi (Basel, Switzerland) · 2026Article
- Establishment of the Genetic Transformation System ofJournal of fungi (Basel, Switzerland) · 2026Article
- The Effector SIX8 Contributes to Virulence of Fusarium oxysporum f. sp. lactucae Race 4 on Lettuce.Molecular plant pathology · 2026Article
- Trans-Kingdom dsRNA Sensing:bioRxiv : the preprint server for biology · 2026Article
- Engineered CRISPR-Cas9 Vector for Efficient Agrobacterium-Mediated Transformation of Phanerochaete chrysosporium.Molecular biotechnology · 2026Article
- Elucidation of the Biological Function and Early-Infection Cell Cycle Regulatory Mechanism of Avocado-InfectingFoods (Basel, Switzerland) · 2026Article
- The visualizable and marker-free gene editing platform mediated by CRISPR/Cas9 in Coleophoma empetri.Applied microbiology and biotechnology · 2026Article
- Harnessing Plant-Microorganism Interactions to Mitigate Biotic and Abiotic Stresses for Sustainable Crops.Plants (Basel, Switzerland) · 2026Review
- Refining the Study of Endophytic Fungi: Best Practices in Antibacterial Research.Current microbiology · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Establishment of a protoplast transformation method for the oleaginous yeast Rhodotorula toruloides.FEMS yeast research · 2026Article
- Gradients of Aliveness and Engineering: A Taxonomy of Fungal Engineered Living Materials.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Construction of a randomly barcoded insertional mutant library in the filamentous fungusbioRxiv : the preprint server for biology · 2025Article
- Establishment of an efficient Agrobacterium tumefaciens-mediated transformation system for an Armillaria species, a host of the fully mycoheterotrophic plant Gastrodia elata.Folia microbiologica · 2025Article
- Providing a toolbox for genomic engineering ofMicrobiology spectrum · 2025Article
- Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.Analytica chimica acta · 2025Article
- Engineering of Global Transcriptional Regulators (GTRs) inJournal of fungi (Basel, Switzerland) · 2025Review
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.