ArticleProceedings of the National Academy of Sciences of the United States of America2017
Detection of isolated protein-bound metal ions by single-particle cryo-STEM.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 59 citations in OpenAlex.
- Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging.Nature methods · 2026Article
- Ferritin Cage-Alginate Oligosaccharide-Stabilized Emulsion for Co-Carriage and Protection of Zinc Ion and Hydrophobic Molecule.Foods (Basel, Switzerland) · 2026Article
- Granzyme B-mimetic nanozyme for nanovesicle targeted anticancer applications.Nature communications · 2026Article
- Subcellular localisation and identification of single atoms using quantitative scanning transmission electron microscopy.Journal of microscopy · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.Nature methods · 2024Article
- Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution.Nature communications · 2024Article
- Charting the molecular landscape of the cell.Structure (London, England : 1993) · 2023Review
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- Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.Nature methods · 2022Article
- Article
- Nondestructive production of exosomes loaded with ultrathin palladium nanosheets for targeted bio-orthogonal catalysis.Nature protocols · 2021Article
- Three-dimensional deconvolution processing for STEM cryotomography.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Importance of potassium ions for ribosome structure and function revealed by long-wavelength X-ray diffraction.Nature communications · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Metal ions play essential roles in many aspects of biological chemistry. Detecting their presence and location in proteins and cells is important for understanding biological function. Conventional structural methods such as X-ray crystallography and cryo-transmission electron microscopy can identify metal atoms on protein only if the protein structure is solved to atomic resolution. We demonstrate here the detection of isolated atoms of Zn and Fe on ferritin, using cryogenic annular dark-field scanning transmission electron microscopy (cryo-STEM) coupled with single-particle 3D reconstructions. Zn atoms are found in a pattern that matches precisely their location at the ferroxidase sites determined earlier by X-ray crystallography. By contrast, the Fe distribution is smeared along an arc corresponding to the proposed path from the ferroxidase sites to the mineral nucleation sites along the twofold axes. In this case the single-particle reconstruction is interpreted as a probability distribution function based on the average of individual locations. These results establish conditions for detection of isolated metal atoms in the broader context of electron cryo-microscopy and tomography.
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