ArticleProceedings of the National Academy of Sciences of the United States of America2017
Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 102 citations in OpenAlex.
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- The effect of morning vs evening exercise training on glycaemic control and serum metabolites in overweight/obese men: a randomised trial.Diabetologia · 2021Trial
- Immediate post-breakfast physical activity improves interstitial postprandial glycemia: a comparison of different activity-meal timings.Pflugers Archiv : European journal of physiology · 2020Trial
- Stephanoside B modulates metabolic gene expression and lengthens the circadian bmal1 oscillation period in differentiated myotubes revealed by real-time bioluminescence.Molecular biology reports · 2026Article
- Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion.Nature metabolism · 2026Article
- Effects of Chrono-Exercise and Chrono-Nutrition on Muscle Health: Understanding the Molecular Mechanisms Activated by Timed Exercise and Consumption of Proteins and Carbohydrates.Nutrition reviews · 2025Review
- Physiological rhythms and metabolic regulation: Shining light on skeletal muscle.Experimental physiology · 2025Review
- Circadian ontogenetic metabolomics atlas: an interactive resource with insights from rat plasma, tissues, and feces.Cellular and molecular life sciences : CMLS · 2025Article
- Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions.The Journal of clinical endocrinology and metabolism · 2025Article
- Liver as a nexus of daily metabolic cross talk.International review of cell and molecular biology · 2025Review
- Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep (Animals : an open access journal from MDPI · 2024Article
- Adipocyte sphingosine kinase 1 regulates histone modifiers to disrupt circadian function.bioRxiv : the preprint server for biology · 2024Article
- Treadmill training impacts the skeletal muscle molecular clock after ischemia stroke in rats.Heliyon · 2024Article
- Circadian organization of lipid landscape is perturbed in type 2 diabetic patients.Cell reports. Medicine · 2023Article
- Metabolism and exercise: the skeletal muscle clock takes centre stage.Nature reviews. Endocrinology · 2023Review
- The Effect of Night Shifts on 24-h Rhythms in the Urinary Metabolome of Police Officers on a Rotating Work Schedule.Journal of biological rhythms · 2023Article
- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets.PLoS biology · 2022Article
- The role of melatonin in the molecular mechanisms underlying metaflammation and infections in obesity: A narrative review.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2022Review
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Authors and funding
18 authors at 7 institutions in 3 countries.
Funding
Abstract
Circadian clocks play an important role in lipid homeostasis, with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism, we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has not been shown whether lipid oscillations in peripheral tissues are driven by diurnal cycles of rest-activity and food intake or are able to persist in vitro in a cell-autonomous manner. To address this, we investigated lipid profiles over 24 h in human skeletal muscle in vivo and in primary human myotubes cultured in vitro. Glycerolipids, glycerophospholipids, and sphingolipids exhibited diurnal oscillations, suggesting a widespread circadian impact on muscle lipid metabolism. Notably, peak levels of lipid accumulation were in phase coherence with core clock gene expression in vivo and in vitro. The percentage of oscillating lipid metabolites was comparable between muscle tissue and cultured myotubes, and temporal lipid profiles correlated with transcript profiles of genes implicated in their biosynthesis. Lipids enriched in the outer leaflet of the plasma membrane oscillated in a highly coordinated manner in vivo and in vitro. Lipid metabolite oscillations were strongly attenuated upon siRNA-mediated clock disruption in human primary myotubes. Taken together, our data suggest an essential role for endogenous cell-autonomous human skeletal muscle oscillators in regulating lipid metabolism independent of external synchronizers, such as physical activity or food intake.
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