ArticleNucleic acids research2017
Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Prophage Activation Triggers Extracellular Synthesis of DNA in Bacterial Biofilms via Rolling Circle Replication.Angewandte Chemie (International ed. in English) · 2026Article
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- Self-contained G-quadruplex/hemin DNAzyme: a superior ready-made catalyst for in situ imaging analysis.Nucleic acids research · 2025Article
- Directed evolution of peroxidase DNAzymes by a function-based approach.Biology methods & protocols · 2025Review
- Article
- Stress promotes RNA G-quadruplex folding in human cells.Nature communications · 2023Article
- Article
- G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes.Genome biology · 2022Article
- Recent advances in RNA structurome.Science China. Life sciences · 2022Review
- Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.Chembiochem : a European journal of chemical biology · 2022Article
- Zippered G-quadruplex/hemin DNAzyme: exceptional catalyst for universal bioanalytical applications.Nucleic acids research · 2021Article
- Oxidation-Induced "One-Pot" Click Chemistry.Chemical reviews · 2021Article
- Phenoxy Radical Reactivity of Nucleic Acids: Practical Implications for Biotinylation.Chembiochem : a European journal of chemical biology · 2021Article
- Properties and biological impact of RNA G-quadruplexes: from order to turmoil and back.Nucleic acids research · 2020Article
- High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.Nucleic acids research · 2020Article
- DNAzymes as Catalysts for l-Tyrosine and Amyloid β Oxidation.ACS omega · 2020Article
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The striking and ubiquitous in vitro affinity between hemin and DNA/RNA G-quadruplexes raises the intriguing possibility of its relevance to biology. To date, no satisfactory experimental framework has been reported for investigating such a possibility. Complexation by G-quadruplexes leads to activation of the bound hemin toward catalysis of 1- and 2-electron oxidative reactions, with phenolic compounds being particularly outstanding substrates. We report here a strategy for exploiting that intrinsic peroxidase activity of hemin•G-quadruplex complexes for self-biotinylation of their G-quadruplex component. Such self-biotinylation occurs with good efficiency and high discrimination in vitro, being specific for G-quadruplexes and not for duplexes. The biotinylated DNA, moreover, remains amenable to polymerase chain reaction amplification, rendering it suitable for analysis by ChIP-Seq and related methods. We anticipate that this self-biotinylation methodology will also serve as a sensitive tool, orthogonal to existing ones, for identifying, labeling and pulling down cellular RNA and DNA G-quadruplexes in general, as well as proteins bound to or proximal to such quadruplexes.
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