ArticleInvestigative ophthalmology & visual science2017
Generation and Characterization of a Novel Mouse Line, Keratocan-rtTA (KeraRT), for Corneal Stroma and Tendon Research.
Article in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Keratocyte Depletion by Genetic Manipulation Re-creates Corneal Ectasia in a Mouse Model.The American journal of pathology · 2026Article
- A Core of Keratocan-Negative Cells Survives in Old Corneal Scars.The American journal of pathology · 2025Article
- SMAD4-Dependent Signaling Pathway Involves in the Pathogenesis of TGFBR2-Related CE-like Phenotype.Cells · 2024Article
- The RBPMSiScience · 2023Article
- Keratocyte-Derived Myofibroblasts: Functional Differences With Their Fibroblast Precursors.Investigative ophthalmology & visual science · 2023Article
- Role of Fibroblast Growth Factor Receptor 2 (FGFR2) in Corneal Stromal Thinning.Investigative ophthalmology & visual science · 2023Article
- Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation.Disease models & mechanisms · 2023Article
- Ablation of collagen XII disturbs joint extracellular matrix organization and causes patellar subluxation.iScience · 2023Article
- Small leucine rich proteoglycans: Biology, function and their therapeutic potential in the ocular surface.The ocular surface · 2023Review
- Genipin increases extracellular matrix synthesis preventing corneal perforation.The ocular surface · 2023Article
- Inducible Slc4a11 Knockout Triggers Corneal Edema Through Perturbation of Corneal Endothelial Pump.Investigative ophthalmology & visual science · 2021Article
- Composition, structure and function of the corneal stroma.Experimental eye research · 2020Review
- Excess Transforming Growth Factor-α Changed the Cell Properties of Corneal Epithelium and Stroma.Investigative ophthalmology & visual science · 2020Article
- Distribution and Function of Glycosaminoglycans and Proteoglycans in the Development, Homeostasis and Pathology of the Ocular Surface.Frontiers in cell and developmental biology · 2020Review
- Primary cilia deficiency in neural crest cells models anterior segment dysgenesis in mouse.eLife · 2019Article
- Aberrant expression of a stabilized β-catenin mutant in keratocytes inhibits mouse corneal epithelial stratification.Scientific reports · 2019Article
- Repressed Wnt Signaling Accelerates the Aging Process in Mouse Eyes.Journal of ophthalmology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Purpose: We created a novel inducible mouse line Keratocan-rtTA (KeraRT) that allows specific genetic modification in corneal keratocytes and tenocytes during development and in adults. Methods: A gene-targeting vector (Kera- IRES2-rtTA3) was constructed and inserted right after the termination codon of the mouse Kera allele via gene targeting techniques. The resulting KeraRT mouse was crossed to tet-O-Hist1H2B-EGFP (TH2B-EGFP) to obtain KeraRT/TH2B-EGFP compound transgenic mice, in which cells expressing Kera are labeled with green fluorescence protein (GFP) by doxycycline (Dox) induction. The expression patterns of GFP and endogenous Kera were examined in KeraRT/TH2B-EGFP. Moreover, KeraRT was bred with tet-O-TGF-α to generate a double transgenic mouse, KeraRT/tet-O-TGF-α, to overexpress TGF-α in corneal keratocytes upon Dox induction. Results: Strong GFP-labeled cells were detected in corneal stroma, limbs, and tail when KeraRT/TH2B-EGFP mice were fed Dox chow. There was no GFP in any single transgenic KeraRT or TH2B-EGFP mouse. Histological analysis showed that GFP in the cornea was limited to stromal keratocytes of KeraRT/TH2B-EGFP, which is consistent with Kera expression. Induction of GFP occurred in 24 hours and reached a plateau by 7 days after Dox induction. GFP could be detected 3-months after induction of KeraRT/TH2B-EGFP. Ectopic expression of TGF-α in corneal keratocytes caused hyperplasia in the corneal epithelium and stroma. Conclusions: The novel Dox inducible KeraRT driver mouse line is a useful genetic tool for gene manipulation and elucidating gene functions in corneal stroma and tendons of limbs and tail during embryonic development, homeostasis and pathogenesis.
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Registered trials
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