Evidence map›Paper›PMID 28972822›Full record

ArticleJournal of dental research2018

Inhibition of TGF-β Signaling in SHED Enhances Endothelial Differentiation.

J G Xu, T Gong, Y Y Wang, T Zou, B C Heng, Y Q Yang, C F Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

  1. Pooled it
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  9. Influencing factors of pulp-dentin complex regeneration and related biological strategies.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

J G Xu1 Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
T Gong1 Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Y Y Wang3 Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
T Zou1 Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
B C Heng1 Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Y Q Yang4 Orthodontics, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
C F Zhang1 Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
University of Hong Kong · HKCity University of Hong Kong, Shenzhen Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low efficiency of deriving endothelial cells (ECs) from adult stem cells hampers their utilization in tissue engineering studies. The purpose of this study was to investigate whether suppression of transforming growth factor beta (TGF-β) signaling could enhance the differentiation efficiency of dental pulp-derived stem cells into ECs. We initially used vascular endothelial growth factor A (VEGF-A) to stimulate 2 dental pulp-derived stem cells (dental pulp stem cells and stem cells from human exfoliated deciduous teeth [SHED]) and compared their differentiation capacity into ECs. We further evaluated whether the vascular endothelial growth factor receptor I (VEGF-RI)-specific ligand placental growth factor-1 (PlGF-1) could mediate endothelial differentiation. Finally, we investigated whether the TGF-β signaling inhibitor SB-431542 could enhance the inductive effect of VEGF-A on endothelial differentiation, as well as the underlying mechanisms involved. ECs differentiated from dental pulp-derived stem cells exhibited the typical phenotypes of primary ECs, with SHED possessing a higher endothelial differentiation potential than dental pulp stem cells. VEGFR1-specific ligand-PLGF exerted a negligible effect on SHED-ECs differentiation. Compared with VEGF-A alone, the combination of VEGF-A and SB-431542 significantly enhanced the endothelial differentiation of SHED. The presence of SB-431542 inhibited the phosphorylation of Suppressor of Mothers Against Decapentaplegic 2/3 (SMAD2/3), allowing for VEGF-A-dependent phosphorylation and upregulation of VEGFR2. Our results indicate that the combination of VEGF-A and SB-431542 could enhance the differentiation of dental pulp-derived stem cells into endothelial cells, and this process is mediated through enhancement of VEGF-A-VEGFR2 signaling and concomitant inhibition of TGF-β-SMAD2/3 signaling.

Indexed as

AdolescentBenzamidesCell DifferentiationCells, CulturedChildDental PulpDioxolesEndothelial CellsHumansMaleMembrane ProteinsPhenotypePhosphorylationSignal TransductionStem CellsTooth, Deciduous4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamideBenzamidesDioxolesMembrane ProteinsPIGF protein, humanTransforming Growth Factor betaVascular Endothelial Growth Factor Aangiogenesis inducing agentscell differentiationdental pulpsignal transductionstem cellsvascular endothelial growth factors

Identifiers

PMID28972822
PMCPMC6429570
OpenAlexW2764001195

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.