ArticleJournal of lipid research2017
Nile Red Quantifier: a novel and quantitative tool to study lipid accumulation in patient-derived circulating monocytes using confocal microscopy.
Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- Mechanistic role of lipid metabolism in foot-and-mouth disease virus (FMDV) replication.Veterinary research · 2026Article
- Mechanism-based new approach methodologies for in vitro detection of chemical-induced liver steatosis.Archives of toxicology · 2026Review
- Hyperspectral coherent anti-Stokes Raman scattering volumetric projection microscopy for assessing drug-cell interaction.Biomedical optics express · 2026Article
- BACE2 tunes lipid uptake through lipid transporters shedding supporting cancer cell proliferation.Journal of experimental & clinical cancer research : CR · 2026Article
- Solanum mauritianum Scop. Leaf Extract Attenuates ROS-Mediated Inflammation and Prevents IL-6 Promoter Demethylation in Activated Monocytes.Applied biochemistry and biotechnology · 2026Article
- Hypercholesterolemia-induced impairment in sorafenib functionality is overcome by avasimibe co-treatment.Journal of biosciences · 2026Article
- NarL gene modulates the host lipid metabolism for survival of Salmonella Typhimurium in murine macrophages.Molecular biology reports · 2025Article
- Diacylglycerols and Lysophosphatidic Acid, Enriched on Lipoprotein(a), Contribute to Monocyte Inflammation.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Poly-Unsaturated Fatty Acids (PUFAs) fromJournal of fungi (Basel, Switzerland) · 2024Article
- Enterovirus A71 utilizes host cell lipid β-oxidation to promote its replication.Frontiers in microbiology · 2022Article
- Antioxidant and lipid-reducing effects ofFood science and biotechnology · 2022Article
- Cytotoxic activity of cholesterol oxidase produced by Streptomyces sp. AKHSS against cancerous cell lines: mechanism of action in HeLa cells.World journal of microbiology & biotechnology · 2021Article
- BET protein inhibitor apabetalone (RVX-208) suppresses pro-inflammatory hyper-activation of monocytes from patients with cardiovascular disease and type 2 diabetes.Clinical epigenetics · 2020Article
- Atherogenic Lipoprotein(a) Increases Vascular Glycolysis, Thereby Facilitating Inflammation and Leukocyte Extravasation.Circulation research · 2020Article
- Inhibition of PFKFB3 Hampers the Progression of Atherosclerosis and Promotes Plaque Stability.Frontiers in cell and developmental biology · 2020Article
- Ultrasound-Assisted Encapsulation of Sacha Inchi (Polymers · 2019Article
- Lipid droplet quantification based on iterative image processing.Journal of lipid research · 2019Article
- Recent Advances in Fluorescent Probes for Lipid Droplets.Materials (Basel, Switzerland) · 2018Review
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inflammatory profile of circulating monocytes is an important biomarker for atherosclerotic plaque vulnerability. Recent research revealed that peripheral lipid uptake by monocytes alters their phenotype toward an inflammatory state and this coincides with an increased lipid droplet (LD) content. Determination of lipid content of circulating monocytes is, however, not very well established. Based on Nile Red (NR) neutral LD imaging, using confocal microscopy and computational analysis, we developed NR Quantifier (NRQ), a novel quantification method to assess LD content in monocytes. Circulating monocytes were isolated from blood and used for the NR staining procedure. In monocytes stained with NR, we clearly distinguished, based on 3D imaging, phospholipids and exclusively intracellular neutral lipids. Next, we developed and validated NRQ, a semi-automated quantification program that detects alterations in lipid accumulation. NRQ was able to detect LD alterations after ex vivo exposure of isolated monocytes to freshly isolated LDL in a time- and dose-dependent fashion. Finally, we validated NRQ in patients with familial hypercholesterolemia and obese subjects in pre- and postprandial state. In conclusion, NRQ is a suitable tool to detect even small differences in neutral LD content in circulating monocytes using NR staining.
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