Evidence map›Paper›PMID 28971534›Full record

ArticleMolecular microbiology2017

The Zap1 transcriptional activator negatively regulates translation of the RTC4 mRNA through the use of alternative 5' transcript leaders.

Amanda J Bird, Simon Labbé

Open access · bronzeAbstract readEditorial
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Amanda J BirdDepartments of Human Nutrition and Molecular Genetics, The Ohio State University, 1787 Neil Avenue, Columbus, OH 43210, USA.ORCID http://orcid.org/0000-0002-1846-7050
Simon LabbéDépartement de Biochimie, Faculté de médecine et des sciences de la santé, Pavillon Z-8, 3201, Jean Mignault, Sherbrooke, QC J1E 4K8, Canada.
The Ohio State University · US

Funding

Zinc-dependent regulation of gene expressionR01GM105695 · NIGMS · OHIO STATE UNIVERSITY · PI BIRD, AMANDA JANE · 2014 to 2018
$1.4M
NIGMS NIH HHS R01 GM105695
6 · The paper itself

Abstract

The zinc-responsive transcription activator Zap1 plays a central role in zinc homeostasis in the budding yeast Saccharomyces cerevisiae. In zinc-deficient cells, Zap1 binds to zinc responsive elements in target gene promoters and activates gene expression. In most cases, Zap1-dependent gene activation results in increased levels of mRNAs and proteins. However, Zap1-dependent activation of RTC4 results in increased levels of the RTC4 mRNA and decreased levels of the Rtc4 protein. This atypical regulation results from Zap1-mediated changes in the transcriptional start site for RTC4 and the production of a RTC4 transcript with a longer 5' leader. This long RTC4 transcript contains small upstream open reading frames that prevent translation of the downstream RTC4 ORF. The new studies with Zap1 highlight how a transcriptional activator can facilitate decreased protein expression.

Indexed as

Alternative SplicingGene Expression Regulation, FungalPromoter Regions, GeneticRepressor ProteinsRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-ActivatorsTranscriptional ActivationTranscription FactorsTranscription, GeneticTranscription Initiation SiteZincRepressor ProteinsRNA, MessengerSaccharomyces cerevisiae ProteinsTrans-ActivatorsTranscription FactorsZAP1 protein, S cerevisiaeZinc

Identifiers

PMID28971534
PMCPMC5705029
OpenAlexW2764246165

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.