Evidence map›Paper›PMID 28970970›Full record

ArticlePeerJ2017

A probe-based qRT-PCR method to profile immunological gene expression in blood of captive beluga whales (

Ming-An Tsai, I-Hua Chen, Jiann-Hsiung Wang, Shih-Jen Chou, Tsung-Hsien Li, Ming-Yih Leu, Hsiao-Kuan Ho, Wei Cheng Yang

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ming-An Tsai *Department of Biology, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
I-Hua Chen *College of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Jiann-Hsiung WangCollege of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Shih-Jen ChouCollege of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Tsung-Hsien LiDepartment of Biology, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
Ming-Yih LeuDepartment of Biology, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
Hsiao-Kuan HoDepartment of Biology, Hi-Scene World Enterprise Co. Ltd., Pingtung, Taiwan.
Wei Cheng YangCollege of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
National Chiayi University · TWNational Museum of Marine Biology and Aquarium · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytokines are fundamental for a functioning immune system, and thus potentially serve as important indicators of animal health. Quantitation of mRNA using quantitative reverse transcription polymerase chain reaction (qRT-PCR) is an established immunological technique. It is particularly suitable for detecting the expression of proteins against which monoclonal antibodies are not available. In this study, we developed a probe-based quantitative gene expression assay for immunological assessment of captive beluga whales (

Indexed as

BelugaGene expressionImmunologyqRT-PCR

Identifiers

PMID28970970
PMCPMC5622604
OpenAlexW2759613173

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.