ArticlePeerJ2017
Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studies.
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
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- 3D printed hybrid scaffolds do not induce adverse inflammation in mice and direct human BM-MSC chondrogenesisBiomaterials and biosystems · 2024Article
- Associations between Cerebrovascular Function and the Expression of Genes Related to Endothelial Function in Hormonal Migraine.International journal of molecular sciences · 2024Article
- Potential association between obstructive lung diseases and cognitive decline.Frontiers in immunology · 2024Observational
- Co-administration of Chrysin and Luteolin with Cisplatin and Topotecan Exhibits a Variable Therapeutic Value in Human Cancer Cells, HeLa.ACS omega · 2023Article
- GAPDH: unveiling its impact as a key hypoxia-related player in head and neck squamous cell carcinoma tumor progression, prognosis, and therapeutic potential.American journal of cancer research · 2023Article
- Candidate genes and sequence variants for susceptibility to mycobacterial infection identified by whole-exome sequencing.Frontiers in genetics · 2022Article
- Validation of Reference Genes via qRT-PCR in Multiple Conditions in Brandt's Voles,Animals : an open access journal from MDPI · 2021Article
- Integrated analysis of mRNA and protein expression profiling in tubal endometriosis.Reproduction (Cambridge, England) · 2020Article
- Identification and Validation of Reference Genes Selection in Ovarian Cancer Exposed to Hypoxia.OncoTargets and therapy · 2020Article
- Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models.Cell death & disease · 2018Article
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTibetans have lived at high altitudes for thousands of years, and they have unique physiological traits that enable them to tolerate this hypoxic environment. However, the genetic basis of these traits is still unknown. As a sensitive and highly efficient technique, RT-qPCR is widely used in gene expression analyses to provide insight into the molecular mechanisms underlying environmental changes. However, the quantitative analysis of gene expression in blood is limited by a shortage of stable reference genes for the normalization of mRNA levels. Thus, systematic approaches were used to identify potential reference genes.
resultsThe expression levels of eight candidate human reference genes (
conclusionThese results indicate that different reference genes should be selected for the normalization of gene expression in blood from different environmental settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.