ArticleChemical science2017
A simple and traceless solid phase method simplifies the assembly of large peptides and the access to challenging proteins.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Covalent capture and genetic code expansion enables chemoproteomic profiling and functional characterization of lysine acetoacetylation.Science advances · 2026Article
- Advances in the chemical synthesis of human proteoforms.Science China. Life sciences · 2025Review
- Amide Chemistry Enables Redox Locking of Cyclic Disulfides for Polypeptide Assembly.Angewandte Chemie (International ed. in English) · 2025Article
- Unlocking the power of antimicrobial peptides: advances in production, optimization, and therapeutics.Frontiers in cellular and infection microbiology · 2025Review
- Development of Convergent Hybrid Phase Ligation for Efficient and Convenient Total Synthesis of Proteins.Peptide science (Hoboken, N.J.) · 2023Article
- Towards the use of an amino acid cleavable linker for solid-phase chemical synthesis of peptides and proteins.Organic & biomolecular chemistry · 2023Article
- Redox-Controlled Chemical Protein Synthesis: Sundry Shades of Latency.Accounts of chemical research · 2022Article
- Insights into the Mechanism and Catalysis of Peptide Thioester Synthesis by Alkylselenols Provide a New Tool for Chemical Protein Synthesis.Molecules (Basel, Switzerland) · 2021Article
- Traceless parallel peptide purification by a first-in-class reductively cleavable linker system featuring a safety-release.Chemical science · 2021Article
- On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins.Journal of the American Chemical Society · 2020Article
- Solid-Phase Synthesis of Megamolecules.Journal of the American Chemical Society · 2020Article
- Solid-Phase Synthesis of Head to Side-Chain Tyr-Cyclodepsipeptides Through a Cyclative Cleavage From Fmoc-MeDbz/MeNbz-resins.Frontiers in chemistry · 2020Article
- Advancing the Frontiers of Chemical Protein Synthesis-The 7Cell chemical biology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical protein synthesis gives access to well-defined native or modified proteins that are useful for studying protein structure and function. The majority of proteins synthesized up to now have been produced using native chemical ligation (NCL) in solution. Although there are significant advantages to assembling large peptides or proteins by solid phase ligation, reports of such approaches are rare. We report a novel solid phase method for protein synthesis which relies on the chemistry of the acetoacetyl group and ketoxime ligation for the attachment of the peptide to the solid support, and on a tandem transoximation/rearrangement process for the detachment of the target protein. Importantly, we show that the combination of solid phase and solution ligation techniques facilitates the production of a challenging and biologically active protein made of 180 amino acids. We show also that the solid phase method enables the purification of complex peptide segments through a chemoselective solid phase capture/release approach.
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