Evidence map›Paper›PMID 28970844›Full record

ReviewFrontiers in plant science2017

CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops.

Suhas G Karkute, Achuit K Singh, Om P Gupta, Prabhakar M Singh, Bijendra Singh

Abstract readReview
In one paragraph

Review in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. CRISPR/Cas9-Mediated Editing ofFrontiers in plant science · 2022
    Article
  16. A glance at the chemodiversity ofPhytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2022
    Review
  17. Review
  18. Article
  19. Impacts of the regulatory environment for gene editing on delivering beneficial products.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association · 2021
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Suhas G KarkuteDivision of Vegetable Improvement, ICAR-Indian Institute of Vegetable ResearchVaranasi, India.
Achuit K SinghDivision of Vegetable Improvement, ICAR-Indian Institute of Vegetable ResearchVaranasi, India.
Om P GuptaDivision of Quality and Basic Sciences, ICAR-Indian Institute of Wheat and Barley ResearchKarnal, India.
Prabhakar M SinghDivision of Vegetable Improvement, ICAR-Indian Institute of Vegetable ResearchVaranasi, India.
Bijendra SinghDivision of Vegetable Improvement, ICAR-Indian Institute of Vegetable ResearchVaranasi, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Horticultural crops are an important part of agriculture for food as well as nutritional security. However, several pests and diseases along with adverse abiotic environmental factors pose a severe threat to these crops by affecting their quality and productivity. This warrants the effective and accelerated breeding programs by utilizing innovative biotechnological tools that can tackle aforementioned issues. The recent technique of genome editing by Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) has greatly advanced the breeding for crop improvement due to its simplicity and high efficiency over other nucleases such as Zinc Finger Nucleases and Transcription Activator Like Effector Nucleases. CRISPR/Cas9 tool contains a non-specific Cas9 nuclease and a single guide RNA that directs Cas9 to the specific genomic location creating double-strand breaks and subsequent repair process creates insertion or deletion mutations. This is currently the widely adopted tool for reverse genetics, and crop improvement in large number of agricultural crops. The use of CRISPR/Cas9 in horticultural crops is limited to few crops due to lack of availability of regeneration protocols and sufficient sequence information in many horticultural crops. In this review, the present status of applicability of CRISPR/Cas9 in horticultural crops was discussed along with the challenges and future potential for possible improvement of these crops for their yield, quality, and resistance to biotic and abiotic stress.

Indexed as

CRISPR/Cas9fruitsgenome editinghorticultural cropsvegetables

Identifiers

PMID28970844
PMCPMC5609112

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.