ArticleScientific reports2017
Identification and characterization of the novel colonization factor CS30 based on whole genome sequencing in enterotoxigenic Escherichia coli (ETEC).
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Emerging and Underdetected Colonization Factors in EnterotoxigenicMicroorganisms · 2026Review
- One Health Perspective on the EnterotoxigenicMicroorganisms · 2026Review
- Beyond FimH: Diversity and Relevance of Carbohydrate-Binding Fimbrial Proteins in Escherichia coli.Chembiochem : a European journal of chemical biology · 2025Review
- Genomic profiling of enterotoxigenicMicrobial genomics · 2025Article
- Genome Sequencing and Assembly of EnterotoxigenicMicroorganisms · 2025Article
- Glycan-mediated adhesion mechanisms in antibiotic-resistant bacteria.BBA advances · 2025Article
- Construction of the ETECFinder database for the characterization of enterotoxigenicJournal of clinical microbiology · 2024Article
- Inactivation ofHeliyon · 2024Article
- Article
- Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of EnterotoxigenicInternational journal of molecular sciences · 2022Article
- Intestinal Epithelial Cells Modulate the Production of Enterotoxins by Porcine EnterotoxigenicInternational journal of molecular sciences · 2022Article
- Functional analysis of colonization factor antigen I positive enterotoxigenicMicrobial genomics · 2021Article
- Long-read-sequenced reference genomes of the seven major lineages of enterotoxigenic Escherichia coli (ETEC) circulating in modern time.Scientific reports · 2021Article
- Genomic Epidemiology and Antimicrobial Susceptibility Profile of EnterotoxigenicFrontiers in microbiology · 2021Article
- Colonization factor CS30 from enterotoxigenicVirulence · 2020Article
- Conservation and global distribution of non-canonical antigens in Enterotoxigenic Escherichia coli.PLoS neglected tropical diseases · 2019Article
- Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile.PLoS neglected tropical diseases · 2019Article
- Enterotoxigenic Escherichia coli Infections.Current infectious disease reports · 2019Review
- The bile salt glycocholate induces global changes in gene and protein expression and activates virulence in enterotoxigenic Escherichia coli.Scientific reports · 2019Article
- Heat-Stable Enterotoxins of EnterotoxigenicToxins · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to colonize the small intestine is essential for enterotoxigenic Escherichia coli (ETEC) to cause diarrhea. Although 22 antigenically different colonization factors (CFs) have been identified and characterized in ETEC at least 30% of clinical ETEC isolates lack known CFs. Ninety-four whole genome sequenced "CF negative" isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyses. We identified a novel CF, CS30, encoded by a set of seven genes, csmA-G, related to the human CF operon CS18 and the porcine CF operon 987P (F6). CS30 was shown to be thermo-regulated, expressed at 37 °C, but not at 20 °C, by SDS-page and mass spectrometry analyses as well as electron microscopy imaging. Bacteria expressing CS30 were also shown to bind to differentiated human intestinal Caco-2 cells. The genes encoding CS30 were located on a plasmid (E873p3) together with the genes encoding LT and STp. PCR screening of ETEC isolates revealed that 8.6% (n = 13) of "CF negative" (n = 152) and 19.4% (n = 13) of "CF negative" LT + STp (n = 67) expressing isolates analyzed harbored CS30. Hence, we conclude that CS30 is common among "CF negative" LT + STp isolates and is associated with ETEC that cause diarrhea.
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