ArticleScientific reports2017
DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- DNA Barcoding and Comparative Chloroplast Marker Performance in Endemic Plants of Crete (Greece).Current issues in molecular biology · 2026Article
- Phylogenetic relationship based on DNA barcodes and comparative analysis of phytochemical contents among Rhynchostylis orchids in Thailand.Scientific reports · 2026Article
- Contrasting habitat associations and ecophysiological adaptations drive interspecific growth differences among Himalayan high-mountain plants.Annals of botany · 2025Article
- Using RAD-seq to identify and differentiate the medicinal herb Scrophularia ningpoensis and its adulterants.BMC genomics · 2025Article
- Insights from DNA Barcodes-Based Phylogenetic Analysis of Medicinal Plants and Estimation of Their Conservation Status: A Case Study in the Tianshan Wild Forest, China.Plants (Basel, Switzerland) · 2025Article
- Internal transcribed spacer (ITS): The powerful DNA barcode and phylogenetic marker for successful authentication of Withania somnifera.Molecular biology reports · 2024Article
- Molecular identification, genetic diversity, and secondary structure predictions of Physalis species using ITS2 DNA barcoding.BMC plant biology · 2024Article
- Authentication of important medicinal herbal species through DNA-based molecular characterization.Saudi journal of biological sciences · 2024Article
- Exploring the Phytochemical Composition and Biological Potential of Balkan Endemic SpeciesPlants (Basel, Switzerland) · 2023Article
- Genetic diversity among local mango (Mangifera indica L.) germplasm using morphological, biochemical and chloroplast DNA barcodes analyses.Molecular biology reports · 2022Article
- A Review on Application of DNA Barcoding Technology for Rapid Molecular Diagnostics of Adulterants in Herbal Medicine.Drug safety · 2022Review
- Molecular identification and phylogenetic analysis ofSaudi journal of biological sciences · 2022Article
- Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.BMC plant biology · 2022Article
- Genetic relationship and source species identification of 58 Qi-Nan germplasms of Aquilaria species in China that easily form agarwood.PloS one · 2022Article
- Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data.Insects · 2021Article
- Comparison of matK and rbcL DNA barcodes for genetic classification of jewel orchid accessions in Vietnam.Journal, genetic engineering & biotechnology · 2021Article
- Essential Oil Composition and DNA Barcode and Identification ofMolecules (Basel, Switzerland) · 2021Article
- Phylogenetic relationships and DNA barcoding of nine endangered medicinal plant species endemic to Saint Katherine protectorate.Saudi journal of biological sciences · 2021Article
- Molecular characterization of endangered endemic plantSaudi journal of biological sciences · 2021Article
- In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate.Journal, genetic engineering & biotechnology · 2020Article
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Authors and funding
4 authors.
Funding
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Abstract
DNA barcoding is a useful tool for species identification and phylogenetic construction. But present studies have far reached a consistent result on the universality of DNA barcoding. We tested the universality of tree species DNA barcodes including rbcL, matK, trnH-psbA and ITS, and examined their abilities of species identification and phylogenetic construction in three tropical cloud forests. Results showed that the success rates of PCR amplification of rbcL, matK, trnH-psbA and ITS were 75.26% ± 3.65%, 57.24% ± 4.42%, 79.28% ± 7.08%, 50.31% ± 6.64%, and the rates of DNA sequencing were 63.84% ± 4.32%, 50.82% ± 4.36%, 72.87% ± 11.37%, 45.15% ± 8.91% respectively, suggesting that both rbcL and trnH-psbA are universal for tree species in the tropical cloud forests. The success rates of species identification of the four fragments were higher than 41.00% (rbcL: 41.50% ± 2.81%, matK: 42.88% ± 2.59%, trnH-psbA: 46.16% ± 5.11% and ITS: 47.20% ± 5.76%), demonstrating that these fragments have potentiality in species identification. When the phylogenetic relationships were built with random fragment combinations, optimal evolutionary tree with high supporting values were established using the combinations of rbcL + matK + trnH-psbA in tropical cloud forests.
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