ArticleParticle and fibre toxicology2017
In vitro toxicoproteomic analysis of A549 human lung epithelial cells exposed to urban air particulate matter and its water-soluble and insoluble fractions.
Article in Particle and fibre toxicology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 71 citations in OpenAlex.
- Epigenetics and environmental health.Frontiers of medicine · 2024Review
- Inter-continental variability in the relationship of oxidative potential and cytotoxicity with PMNature communications · 2024Article
- Surface property andEco-Environment & Health · 2024Article
- Assessing the cytotoxicity of aerosolized carbon black and benzo[a]pyrene with controlled physical and chemical properties on human lung epithelial cells.Scientific reports · 2023Article
- Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro.Nanomaterials (Basel, Switzerland) · 2022Article
- Potential cytotoxicity of PM2.5-bound PAHs and toxic metals collected from areas with different traffic densities on human lung epithelial cells (A549).Journal of environmental health science & engineering · 2021Article
- In vitro impact preliminary assessment of airborne particulate from metalworking and woodworking industries.Scientific reports · 2021Article
- Effects of water-soluble components of atmospheric particulates from rare earth mining areas in China on lung cancer cell cycle.Particle and fibre toxicology · 2021Article
- Prolonged exposure to traffic-related particulate matter and gaseous pollutants implicate distinct molecular mechanisms of lung injury in rats.Particle and fibre toxicology · 2021Article
- Toxicity of Water- and Organic-Soluble Wood Tar Fractions from Biomass Burning in Lung Epithelial Cells.Chemical research in toxicology · 2021Article
- GerminatedInternational journal of molecular sciences · 2021Article
- Urban Particles Elevated Streptococcus pneumoniae Biofilms, Colonization of the Human Middle Ear Epithelial Cells, Mouse Nasopharynx and Transit to the Middle Ear and Lungs.Scientific reports · 2020Article
- Effect of ambient air pollution on premature SGA in Changzhou city, 2013-2016: a retrospective study.BMC public health · 2019Article
- Amino-PAHs activated Nrf2/ARE anti-oxidative defense system and promoted inflammatory responses: the regulation of PI3K/Akt pathway.Toxicology research · 2018Article
- A critical review of assays for hazardous components of air pollution.Free radical biology & medicine · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxicity of airborne particulate matter (PM) is difficult to assess because PM composition is complex and variable due to source contribution and atmospheric transformation. In this study, we used an in vitro toxicoproteomic approach to identify the toxicity mechanisms associated with different subfractions of Ottawa urban dust (EHC-93).
methodsA549 human lung epithelial cells were exposed to 0, 60, 140 and 200 μg/cm
resultsThe cytotoxicity data based on cellular ATP, BrdU incorporation and LDH leakage indicated that the insoluble, but not the soluble, fraction is responsible for the toxicity of EHC-93 in A549 cells. Two-dimensional gel electrophoresis results revealed that the expressions of 206 protein spots were significantly altered after particle exposures, where 154 were identified by MALDI-TOF-TOF-MS/MS. The results from cytotoxicity assays and proteomic analyses converged to a similar finding that the effects of the total and insoluble fraction may be alike, but their effects were distinguishable, and their effects were significantly different from the soluble fraction. Furthermore, the toxic potency of EHC-93 total is not equal to the sum of its insoluble and soluble fractions, implying inter-component interactions between insoluble and soluble materials resulting in synergistic or antagonistic cytotoxic effects. Pathway analysis based on the low toxicity dose (60 μg/cm
conclusionsThis study demonstrated the impact of different fractions of urban air particles constituted of various chemical species on different mechanistic pathways and thus on cytotoxicity effects. In vitro toxicoproteomics can be a valuable tool in mapping these differences in air pollutant exposure-related toxicity mechanisms.
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