ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.
Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Serological and Molecular Prevalence of HCMV, HCV, HBV andCurrent HIV research · 2026Article
- Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene.BMC infectious diseases · 2022Article
- The Role of microRNAs in the Infection byFrontiers in cellular and infection microbiology · 2021Review
- Is Real-Time PCR Targeting Rep 529 Suitable for Diagnosis of Toxoplasmosis in Patients Infected with Non-Type II Strains in North America?Journal of clinical microbiology · 2020Article
- Human extracellular vesicles and correlation with two clinical forms of toxoplasmosis.PloS one · 2020Article
- Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Biological Diagnosis of Ocular Toxoplasmosis: a Nine-Year Retrospective Observational Study.mSphere · 2019Observational
- Evaluation of Serological and Molecular Tests Used for the Identification ofFrontiers in cellular and infection microbiology · 2019Article
- Serum IgG Anti-Frontiers in cellular and infection microbiology · 2019Article
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Authors and funding
10 authors.
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Abstract
Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in C
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