ArticleJournal of proteome research2017
Elucidating Escherichia coli Proteoform Families Using Intact-Mass Proteomics and a Global PTM Discovery Database.
Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- Intact Mass Proteomics Using a Proteoform Atlas.Journal of proteome research · 2025Article
- Spectral averaging with outlier rejection algorithms to increase identifications in top-down proteomics.Proteomics · 2024Article
- Top-down proteomics.Nature reviews. Methods primers · 2024Article
- Cysteine Counting via Isotopic Chemical Labeling for Intact Mass Proteoform Identifications in Tissue.Analytical chemistry · 2023Article
- Recent advances (2019-2021) of capillary electrophoresis-mass spectrometry for multilevel proteomics.Mass spectrometry reviews · 2023Review
- Revealing Corynebacterium glutamicum proteoforms through top-down proteomics.Scientific reports · 2023Article
- A Hybrid Spectral Library and Protein Sequence Database Search Strategy for Bottom-Up and Top-Down Proteomic Data Analysis.Journal of proteome research · 2022Article
- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Proteoforms and Proteoform Families: Past, Present, and Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics.Journal of the American Society for Mass Spectrometry · 2021Article
- Spritz: A Proteogenomic Database Engine.Journal of proteome research · 2021Article
- Construction of Human Proteoform Families from 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Top-Down Proteomic Data.Journal of proteome research · 2021Article
- Article
- Improving Proteoform Identifications in Complex Systems Through Integration of Bottom-Up and Top-Down Data.Journal of proteome research · 2020Article
- Quick microbial molecular phenotyping by differential shotgun proteomics.Environmental microbiology · 2020Review
- Capillary zone electrophoresis-mass spectrometry for top-down proteomics.Trends in analytical chemistry : TRAC · 2019Article
- Constructing Human Proteoform Families Using Intact-Mass and Top-Down Proteomics with a Multi-Protease Global Post-Translational Modification Discovery Database.Journal of proteome research · 2019Article
- Intact-Mass Analysis Facilitating the Identification of Large Human Heart Proteoforms.Analytical chemistry · 2019Article
- Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing CyanobacteriumBiomolecules · 2019Article
- Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A proteoform family is a group of related molecular forms of a protein (proteoforms) derived from the same gene. We have previously described a strategy to identify proteoforms and elucidate proteoform families in complex mixtures of intact proteins. The strategy is based upon measurements of two properties for each proteoform: (i) the accurate proteoform intact-mass, measured by liquid chromatography/mass spectrometry (LC-MS), and (ii) the number of lysine residues in each proteoform, determined using an isotopic labeling approach. These measured properties are then compared with those extracted from a catalog of theoretical proteoforms containing protein sequences and localized post-translational modifications (PTMs) for the organism under study. A match between the measured properties and those in the catalog constitutes an identification of the proteoform. In the present study, this strategy is extended by utilizing a global PTM discovery database and is applied to the widely studied model organism Escherichia coli, providing the most comprehensive elucidation of E. coli proteoforms and proteoform families to date.
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