ArticleMicrobial biotechnology2018
Transfer and analysis of Salmonella pdu genes in a range of Gram-negative bacteria demonstrate exogenous microcompartment expression across a variety of species.
Article in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign.ACS synthetic biology · 2025Article
- Bacterial microcompartments as a next-generation metabolic engineering tool: utilizing nature's solution for confining challenging catabolic pathways.Biochemical Society transactions · 2024Review
- Modeling bacterial microcompartment architectures for enhanced cyanobacterial carbon fixation.Frontiers in plant science · 2024Review
- Towards using bacterial microcompartments as a platform for spatial metabolic engineering in the industrially important and metabolically versatileFrontiers in bioengineering and biotechnology · 2024Article
- Monatomic ions influence substrate permeation across bacterial microcompartment shells.Scientific reports · 2023Article
- Identification of genes influencing the evolution ofMicrobial genomics · 2023Article
- Chemical probing provides insight into the native assembly state of a bacterial microcompartment.Structure (London, England : 1993) · 2022Article
- New discoveries expand possibilities for carboxysome engineering.Current opinion in microbiology · 2021Review
- Advances in the World of Bacterial Microcompartments.Trends in biochemical sciences · 2021Review
- Prokaryotic Organelles: Bacterial Microcompartments inEcoSal Plus · 2020Review
- Phenotypes, transcriptome, and novel biofilm formation associated with the ydcI gene.Antonie van Leeuwenhoek · 2020Article
- Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation.Polish journal of microbiology · 2019Article
- Bacterial microcompartments: catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering.BMC biology · 2019Review
- Cargo encapsulation in bacterial microcompartments: Methods and analysis.Methods in enzymology · 2019Article
- Transfer and analysis of Salmonella pdu genes in a range of Gram-negative bacteria demonstrate exogenous microcompartment expression across a variety of species.Microbial biotechnology · 2018Article
- Facile methods for heterologous production of bacterial microcompartments in diverse host species.Microbial biotechnology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial microcompartments (MCPs) are protein organelles that typically house toxic or volatile reaction intermediates involved in metabolic pathways. Engineering bacteria to express exogenous MCPs will allow these cells to gain useful functions involving molecule compartmentalization. We cloned a 38 kb region from the Salmonella enterica serovar Typhimurium genome containing the pdu 1,2 propanediol (1,2 PD) utilization and cob/cbi genes using the FRT-Capture strategy to clone and transfer large genomic segments. We transferred this clone to a range of Gram-negative bacteria and found the clone to be functional for 1,2 PD metabolism in a variety of species including S. Typhimurium Δpdu, Escherichia coli, Salmonella bongori, Klebsiella pneumoniae, Cronobacter sakazakii, Serratia marcescens, and different Pseudomonas species. We successfully isolated MCPs expressed from the clone from several, but not all, of these strains, and we observed this utilizing a range of different media and in the absence of protease inhibitor. We also present a mini-prep protocol that allows rapid, small-scale screening of strains for MCP production. To date, this is the first analysis of cloned, exogenous microcompartment expression across several different Gram-negative backgrounds and provides a foundation for MCP use in a variety of bacterial species using a full, intact clone.
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