Evidence map›Paper›PMID 28966118›Full record

ArticleStem cell reports2017

Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation.

Raghu Ram Edupuganti, Arigela Harikumar, Yair Aaronson, Alva Biran, Badi Sri Sailaja, Malka Nissim-Rafinia, Gajendra Kumar Azad, Malkiel A Cohen, Jung Eun Park, Chikdu S Shivalila and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Pluripotency genes of mammals: a network at work.Frontiers in bioengineering and biotechnology · 2025
    Review
  4. Article
  5. Article
  6. Review
  7. Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022
    Review
  8. Article
  9. Organization of the Pluripotent Genome.Cold Spring Harbor perspectives in biology · 2021
    Review
  10. Article
  11. Article
  12. Chaperones and Beyond as Key Players in Pluripotency Maintenance.Frontiers in cell and developmental biology · 2019
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 3 countries.

Raghu Ram EdupugantiThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Arigela HarikumarThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Yair AaronsonThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Alva BiranThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Badi Sri SailajaThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Malka Nissim-RafiniaThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
Gajendra Kumar AzadThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Malkiel A CohenWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Jung Eun ParkSchool of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
Chikdu S ShivalilaWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Styliani MarkoulakiWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Siu Kwan SzeSchool of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
Rudolf JaenischWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Eran MeshorerThe Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Electronic address: meshorer@huji.ac.il.
Hebrew University of Jerusalem · ILWhitehead Institute for Biomedical Research · USNanyang Technological University · SG

Funding

GENOMIC IMPRINTING AND THE CLONING OF MICER37CA084198 · NCI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2000 to 2008
$9.2M
In Vitro Reprogramming of Somatic Cells into Pluripotent ES-like CellsR37HD045022 · NICHD · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2009 to 2017
$7.5M
An iPSC based platform for functionally assessing genetic and environmental riskR01NS088538 · NINDS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF, MYERS, RICHARD H · 2014 to 2018
$5.0M
Nuclear Cloning and the Reprogramming of the GenomeR01HD045022 · NICHD · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2003 to 2008
$4.7M
NCI NIH HHS R37 CA084198NICHD NIH HHS R01 HD045022NICHD NIH HHS R37 HD045022NINDS NIH HHS R01 NS088538
6 · The paper itself

Abstract

Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage.

Indexed as

Gene Expression Regulation, DevelopmentalPromoter Regions, GeneticAdaptor Proteins, Signal TransducingAnimalsCell DifferentiationCell ProliferationCell SurvivalChromatin Assembly and DisassemblyEmbryonic Stem CellsHistone AcetyltransferasesHistonesMiceMouse Embryonic Stem CellsNeoplasm ProteinsNerve Tissue ProteinsNeural PlateAdaptor Proteins, Signal TransducingHistone AcetyltransferasesHistonesNeoplasm ProteinsNerve Tissue ProteinsOctamer Transcription Factor-3Protein IsoformsSh3kbp1 protein, mouseTATA-binding protein associated factor 250 kDaTATA-Binding Protein Associated FactorsTranscription Factor TFIIDchromatindifferentiationembryonic stem cellsepigeneticshistone chaperonehistone dynamicspluripotencySETTAF-ITAFI

Identifiers

PMID28966118
PMCPMC5639460
OpenAlexW2759236627

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.