ArticleCell stem cell2017
Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells.
Open access · bronzeAbstract read
In one paragraph
Article in Cell stem cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 369 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
0cells of the map it votes in
369citing papers in PubMed, 1 pooled it
29.6field-weighted citation impact, top 1% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
369 citing papers in PubMed, 1 synthesis or guideline pooled it, 583 citations in OpenAlex.
- Old and emerging therapies for childhood interstitial lung disease (chILD): a systematic review.European respiratory review : an official journal of the European Respiratory Society · 2026Pooled it
- Disease-associated mutations directionally modulate epigenetic age in iPSC-derived tissues.bioRxiv : the preprint server for biology · 2026Article
- Endowing Flexibility of Alveolar Type II Cells to Recapitulate Development From Lung Bud to Alveoli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Sustained YAP/TAZ activation promotes aberrant alveolar epithelial cell differentiation and drives persistent fibrotic remodeling.JCI insight · 2026Article
- Stage-adaptive integration of polydopamine promotes human pluripotent stem cell-derived alveolar organoids differentiation and maturation.Materials today. Bio · 2026Article
- Co-optation of the regenerative role of type 2 alveolar cells in distal lung repair in mice by terminal airway epithelial cells in humans.bioRxiv : the preprint server for biology · 2026Article
- Alveolar organoids: from model systems to applications in respiratory diseases.Journal of translational medicine · 2026Review
- Lung organoids and organ-on-a-chip models in respiratory disease research: advances, applications, and challenges.Respiratory research · 2026Review
- Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset.iScience · 2026Article
- MicroRNA-18a-3p as a biomarker for neonatal respiratory distress syndrome and its effects in alveolar epithelial type II cells.BMC immunology · 2026Article
- Disease-associated genetic variants can cause missense effects in tissue-specific protein isoforms.Nature communications · 2026Article
- Disease modelling with in vitro vascularised organoids.Disease models & mechanisms · 2026Review
- Progenitor Resilience and the Early Onset of Chronic Lung Diseases: NHLBI workshop report.American journal of respiratory cell and molecular biology · 2026Article
- Chronic TGFβ1 Signaling Drives Aberrant Alveolar-Basaloid Metaplasia through a KRT17-Stratifin migratory complex.bioRxiv : the preprint server for biology · 2026Article
- Robust generation of distal respiratory airway organoids by an engineered cuboid chip.Journal of nanobiotechnology · 2026Article
- Macrophages orchestrate antiviral defense and epithelial repair in a human iPSC-derived alveolar air-liquid interface.JCI insight · 2026Article
- Long isoforms of the COPD risk gene FAM13A orchestrate human lung epithelial development.American journal of respiratory cell and molecular biology · 2026Article
- Regenerative therapeutics for chronic obstructive pulmonary disease.Pharmacological reviews · 2026Review
- Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.The EMBO journal · 2026Article
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.American journal of physiology. Lung cellular and molecular physiology · 2026Article
309 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
27 authors at 7 institutions in 2 countries.
Anjali JacobCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Michael MorleyPenn Center for Pulmonary Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Finn HawkinsCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Katherine B McCauleyCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
J C JeanCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Hillary HeinsEdward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA.
Cheng-Lun NaDivision of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Timothy E WeaverDivision of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Marall VedaieCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Killian HurleyCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Anne HindsThe Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Scott J RussoPenn Center for Pulmonary Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Seunghyi KookDepartment of Pediatrics, Monroe Carell Jr. Children's Hospital, Vanderbilt University, Nashville, TN 37232, USA.
William ZachariasPenn Center for Pulmonary Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Matthias OchsInstitute of Functional and Applied Anatomy, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), REBIRTH Cluster of Excellence, 30625 Hannover, Germany.
Katrina TraberThe Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Lee J QuintonThe Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Ana CraneCenter for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, Houston, TX 77030, USA.
Brian R DavisCenter for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, Houston, TX 77030, USA.
Frances V WhiteEdward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA.
Jennifer WambachEdward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA.
Jeffrey A WhitsettDivision of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
F Sessions ColeEdward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA.
Edward E MorriseyPenn Center for Pulmonary Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Susan H GuttentagDepartment of Pediatrics, Monroe Carell Jr. Children's Hospital, Vanderbilt University, Nashville, TN 37232, USA.
Michael F BeersPenn Center for Pulmonary Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Darrell N KottonCenter for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA. Electronic address: dkotton@bu.edu.
Boston University · USPenn Center for AIDS Research · USMallinckrodt (United States) · USCincinnati Children's Hospital Medical Center · USBrown Foundation · USMonroe Carell Jr. Children's Hospital · USMedizinische Hochschule Hannover · DE
Funding
Project-005UL1TR001430 · NCATS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI BAIR-MERRITT, MEGAN H, CENTER, DAVID M. · 2015 to 2024
$52.3MTranslational Research Support CoreP30ES013508 · NIEHS · UNIVERSITY OF PENNSYLVANIA · PI Rebecca A Simmons · 2006 to 2026
$35.3MProgenitor Cell Biology Consortium Administrative Coordinating CenterU01HL099997 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI TERRIN, MICHAEL L · 2009 to 2015
$29.9MBIOLOGY OF THE LUNG--MULTIDISCIPLINARY PROGRAMT32HL007035 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton, JOSEPH P MIZGERD · 1985 to 2026
$24.3MEditing Alveolar Progenitor Cells for Correction of Monogenic DiseaseU01HL134745 · NHLBI · CINCINNATI CHILDRENS HOSP MED CTR · PI KOTTON, DARRELL N., MORRISEY, EDWARD E · 2016 to 2022
$8.3MEpithelial stem/progenitor cells as repair agents in diffuse alveolar damageU01HL134766 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHAPMAN, HAROLD A, KOTTON, DARRELL N. · 2016 to 2022
$8.1MDerivation of Transplantable Lung Epithelial Progenitors from iPS CellsR01HL095993 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton · 2009 to 2026
$7.5MMolecular pathways controlling alveolar epithelial remodeling in development and regenerationR01HL132999 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI MORRISEY, EDWARD E · 2016 to 2023
$4.6MA National iPS Cell Network with Deep Phenotyping for Translational ResearchU01TR001810 · NCATS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI GILAD, YOAV, KOTTON, DARRELL N. · 2016 to 2020
$4.4MNRSA Training CoreTL1TR001410 · NCATS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI KOTTON, DARRELL N. · 2015 to 2024
$4.3MThe Biology of LIF During PneumoniaR01HL111449 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI QUINTON, LEE · 2012 to 2020
$3.7MMOLECULAR SIGNALS FOR TRAFFICKING SURFACTANT PROTEIN BR01HL059959 · NHLBI · CHILDREN'S HOSP OF PHILADELPHIA · PI GUTTENTAG, SUSAN H. · 1998 to 2011
$3.4MBLRD VA I01 BX001176NCATS NIH HHS TL1 TR001410NCATS NIH HHS U01 TR001810NCATS NIH HHS UL1 TR001430NHLBI NIH HHS F31 HL129777NHLBI NIH HHS F31 HL134274NHLBI NIH HHS K08 HL130582NHLBI NIH HHS L30 HL138777NHLBI NIH HHS R01 HL059959NHLBI NIH HHS R01 HL095993NHLBI NIH HHS R01 HL111449NHLBI NIH HHS R01 HL119436NHLBI NIH HHS R01 HL122442NHLBI NIH HHS R01 HL128172NHLBI NIH HHS R01 HL132999NHLBI NIH HHS R01 HL136722NHLBI NIH HHS R24 HL123828NHLBI NIH HHS R33 HL120760NHLBI NIH HHS R56 HL059959NHLBI NIH HHS T32 HL007035NHLBI NIH HHS U01 HL099997NHLBI NIH HHS U01 HL134745NHLBI NIH HHS U01 HL134766NIEHS NIH HHS P30 ES013508NIGMS NIH HHS R01 GM108807NIGMS NIH HHS R01 GM120060
Abstract
Lung alveoli, which are unique to air-breathing organisms, have been challenging to generate from pluripotent stem cells (PSCs) in part because there are limited model systems available to provide the necessary developmental roadmaps for in vitro differentiation. Here we report the generation of alveolar epithelial type 2 cells (AEC2s), the facultative progenitors of lung alveoli, from human PSCs. Using multicolored fluorescent reporter lines, we track and purify human SFTPC+ alveolar progenitors as they emerge from endodermal precursors in response to stimulation of Wnt and FGF signaling. Purified PSC-derived SFTPC+ cells form monolayered epithelial "alveolospheres" in 3D cultures without the need for mesenchymal support, exhibit self-renewal capacity, and display additional AEC2 functional capacities. Footprint-free CRISPR-based gene correction of PSCs derived from patients carrying a homozygous surfactant mutation (SFTPB
Indexed as
Cell DifferentiationBase SequenceCell LineCell ProliferationCell Self RenewalCell SeparationEpithelial CellsGene Expression ProfilingGenes, ReporterHumansLung DiseasesModels, BiologicalPluripotent Stem CellsPulmonary AlveoliPulmonary SurfactantsThyroid Nuclear Factor 1NKX2-1 protein, humanPulmonary SurfactantsThyroid Nuclear Factor 1Wnt Proteinsalveolar epithelial cellCRISPRdevelopmentdisease modelingembryonic stem cellsgene editinghuman induced pluripotent stem cellslungsurfactantsurfactant protein B
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